Scientific Genomics Platform
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Dr. Wei Chen
87: Timoféeff-Ressovsky-Haus (Genomzentrum)
Raum: 1.16
Tel. 9406-2995
Fax. 9406-3068
The recent introduction of massively parallel sequencing technology has revolutionized genomic research. These so-called next generation sequencing platforms, such as Roche/454, Illumina/solexa and ABI/Solid system can sequence DNA orders of magnitude faster and at much lower cost than conventional Sanger method. With their incredible sequencing capacity, in additional to genomic DNA sequencing, a variety of functional genomic assays based on this new generation of sequencers have been developed and implemented in our lab, ranging from targeted mutation screening by combining next-gen sequencing technology with sequence capture methods, high throughput characterization of chromosome translocation breakpoints, ChIP-seq, RNA-seq and small RNA sequencing.
MiRNAs are small non-coding RNAs that control the expression of target genes at the posttranscriptional level. Recently, more and more miRNAs have been implicated in a variety of biological processes. Whereas many attention has been focus on finding the target genes regulated by miRNAs, little is known about the system which regulates miRNA expression. Together with our collaborators, we are establishing methods to study both transcriptional and posttranscriptional regulation of miRNA genes.