Recent publications
2012
Ammar I; Gogol-Doering A; Miskey C; Chen W; Cathomen T; Izsvak Z; Ivics Z
Retargeting transposon insertions by the adeno-associated virus Rep protein
Nucleic Acids Research : (2012-04-19)
Ammar I; Izsvak Z; Ivics Z
The sleeping beauty transposon toolbox
Methods in Molecular Biology 859: 229-240 (2012)
Swierczek M; Izsvak Z; Ivics Z
The Sleeping Beauty transposon system for clinical applications
Expert Opinion on Biological Therapy 12 (2): 139-153 (2012-02)
Petrakis S; Rasko T; Mates L; Ivics Z; Izsvak Z; Kouzi-Koliakou K; Koliakos G
Gateway compatible transposon vector to genetically modify human embryonic kidney and adipose-derived stromal cells
Biotechnology Journal : (2012-02-10)
2011
Garrels W; Holler S; Taylor U; Herrmann D; Struckmann C; Klein S; Barg-Kues B; Nowak-Imialek M; Ehling C; Rath D; Ivics Z; Niemann H; Kues WA
Genotype-independent transmission of transgenic fluorophore protein by boar spermatozoa
PLoS ONE 6 (11): e27563 (2011-11-16)
Kolacsek O; Krizsik V; Schamberger A; Erdei Z; Apati A; Varady G; Mates L; Izsvak Z; Ivics Z; Sarkadi B; Orban TI
Reliable transgene-independent method for determining Sleeping Beauty transposon copy numbers
Mobile DNA 2 (1): 5 (2011-03-03)
Grabundzija I; Izsvak Z; Ivics Z
Insertional engineering of chromosomes with Sleeping Beauty transposition: an overview
Methods in Molecular Biology 738: 69-85 (2011)
Jin Z; Maiti S; Huls H; Singh H; Olivares S; Mates L; Izsvak Z; Ivics Z; Lee DA; Champlin RE; Cooper LJ
The hyperactive Sleeping Beauty transposase SB100X improves the genetic modification of T cells to express a chimeric antigen receptor
Gene Therapy 18 (9): 849-856 (2011-09)
Ivics Z; Izsvak Z; Chapman KM; Hamra FK
Sleeping Beauty transposon mutagenesis of the rat genome in spermatogonial stem cells
Methods 53 (4): 356-365 (2011-04)
Moldt B; Miskey C; Staunstrup NH; Gogol-Doering A; Bak RO; Sharma N; Mates L; Izsvak Z; Chen W; Ivics Z; Mikkelsen JG
Comparative genomic integration profiling of Sleeping Beauty transposons mobilized with high efficacy from integrase-defective lentiviral vectors in primary human cells
Molecular Therapy 19 (8): 1499-1510 (2011-08)
Garrels W; Holler S; Ivics Z; Niemann H; Kues WA
Transgenetic expression by cytoplasmic injection of plasmids and transposon-based constructs in mammalian embryos
Zuechtungskunde 83 (1): 47-67 (2011-01)
Ivics Z; Izsvak Z
Nonviral gene delivery with the Sleeping Beauty transposon system
Human Gene Therapy 22 (9): 1043-1051 (2011-09)
Petkov S; Ivics Z; Niemann H
Progress towards the derivation of porcine induced pluripotent stem cells
Reproduction Fertility and Development 24 (1): 284-285 (2011-12)
Garrels W; Mates L; Holler S; Dalda A; Taylor U; Petersen B; Niemann H; Izsvak Z; Ivics Z; Kues WA
Germline transgenic pigs by Sleeping Beauty transposition in porcine zygotes and targeted integration in the pig genome
PLoS ONE 6 (8): e23573 (2011-08-29)
Galla M; Schambach A; Falk CS; Maetzig T; Kuehle J; Lange K; Zychlinski D; Heinz N; Brugman MH; Goehring G; Izsvak Z; Ivics Z; Baum C
Avoiding cytotoxicity of transposases by dose-controlled mRNA delivery
Nucleic Acids Research 39 (16): 7147-7160 (2011-09-01)
Ivics Z; Izsvak Z; Medrano G; Chapman KM; Hamra FK
Sleeping Beauty transposon mutagenesis in rat spermatogonial stem cells
Nature Protocols 6 (10): 1521-1535 (2011-10)
Ivics Z
Editorial [hot topic: recombinase technology for gene therapy (guest editor: zoltan ivics)]
Current Gene Therapy 11 (5): 331 (2011-10-01)
2010
Ivics Z; Izsvak Z
The expanding universe of transposon technologies for gene and cell engineering
Mobile DNA 1 (1): 25 (2010-12-07)
Wang Y; Liska F; Gosele C; Sedova L; Kren V; Krenova D; Ivics Z; Huebner N; Izsvak Z
A novel active endogenous retrovirus family contributes to genome variability in rat inbred strains
Genome Research 20 (1): 19-27 (2010-01)
Grabundzija I; Irgang M; Mates L; Belay E; Matrai J; Gogol-Doering A; Kawakami K; Chen W; Ruiz P; Chuah MK; Vandendriessche T; Izsvak Z; Ivics Z
Comparative analysis of transposable element vector systems in human cells
Molecular Therapy 18 (6): 1200-1209 (2010-06)
Izsvak Z; Froehlich J; Grabundzija I; Shirley JR; Powell HM; Chapman KM; Ivics Z; Hamra FK
Generating knockout rats by transposon mutagenesis in spermatogonial stem cells
Nature Methods 7 (6): 443-445 (2010-06)
de Silva S; Mastrangelo MA; Lotta LT; Burris CA; Izsvak Z; Ivics Z; Bowers WJ
Herpes simplex virus/Sleeping Beauty Vector-based embryonic gene transfer using the HSB5 mutant: loss of apparent transposition hyperactivity in vivo
Human Gene Therapy 21 (11): 1603-1613 (2010-11)
Schebelle L; Wolf C; Stribl C; Javaheri T; Schnuetgen F; Ettinger A; Ivics Z; Hansen J; Ruiz P; von Melchner H; Wurst W; Floss T
Efficient conditional and promoter-specific in vivo expression of cDNAs of choice by taking advantage of recombinase-mediated cassette exchange using FlEx gene traps
Nucleic Acids Research 38 (9): e106 (2010-05)
Schumann GG; Gogvadze EV; Osanai-Futahashi M; Kuroki A; Muenk C; Fujiwara H; Ivics Z; Buzdin AA
Unique functions of repetitive transcriptomes
International Review of Cell and Molecular Biology 285: 115-188 (2010)
Ivics Z; Izsvak Z
Repetitive elements and genome instability
Seminars in Cancer Biology 20 (4): 197-199 (2010-08)
Albieri I; Onorati M; Calabrese G; Moiana A; Biasci D; Badaloni A; Camnasio S; Spiliotopoulos D; Ivics Z; Cattaneo E; Consalez GG
A DNA-transposon-based approach to functional screening in neural stem cells
Journal of Biotechnology 150 (1): 11-21 (2010-10-01)
Izsvak Z; Hackett PB; Cooper LJ; Ivics Z
Translating Sleeping Beauty transposition into cellular therapies: Victories and challenges
BioEssays 32 (9): 756-767 (2010-09)
Belay E; Matrai J; Acosta-Sanchez A; Ma L; Quattrocelli M; Mates L; Sancho-Bru P; Geraerts M; Yan B; Vermeesch J; Rincon MY; Samara-Kuko E; Ivics Z; Verfaillie CM; Sampaolesi M; Izsvak Z; Vandendriessche T; Chuah MK
Novel hyperactive transposons for genetic modification of induced puripotent and adult stem cells: a non-viral paradigm for coaxed differentiation
Stem Cells 28 (10): 1760-1771 (2010-10)
Kuzmin D; Gogvadze E; Kholodenko R; Grzela DP; Mityaev M; Vinogradova T; Kopantzev E; Malakhova G; Suntsova M; Sokov D; Ivics Z; Buzdin A
Novel strong tissue specific promoter for gene expression in human germ cells
BMC Biotechnology 10 (1): 58 (2010-08-17)
2009
Vandendriessche T; Ivics Z; Izsvak Z; Chuah MK
Emerging potential of transposons for gene therapy and generation of induced pluripotent stem cells
Blood 114 (8): 1461-1468 (2009-08-20)
Ivics Z; Li MA; Mates L; Boeke JD; Nagy A; Bradley A; Izsvak Z
Transposon-mediated genome manipulation in vertebrates
Nature Methods 6 (6): 415-422 (2009-06)
Ohlfest JR; Ivics Z; Izsvak Z
Transposable elements as plasmid-based vectors for long-term gene transfer into tumors
Methods in Molecular Biology 542 (Part 2): 105-116 (2009)
Izsvak Z; Chuah MK; VandenDriessche T; Ivics Z
Efficient stable gene transfer into human cells by Sleeping Beauty transposon vectors
Methods 49 (3): 287-297 (2009-11)
Ivics Z
Genomic parasites and genome evolution
Genome Biology 10 (4): 306 (2009)
Xue X; Huang X; Nodland SE; Mates L; Ma L; Izsvak Z; Ivics Z; Lebien TW; McIvor RS; Wagner JE; Zhou X
Stable gene transfer and expression in cord blood-derived CD34+ hematopoietic stem and progenitor cells by a hyperactive Sleeping Beauty transposon system
Blood 114 (7): 1319-1330 (2009-08-13)
Sinzelle L; Izsvak Z; Ivics Z
Molecular domestication of transposable elements: From detrimental parasites to useful host genes
Cellular and Molecular Life Sciences 66 (6): 1073-1093 (2009-03)
Orban TI; Apati A; Nemeth A; Varga N; Krizsik V; Schamberger A; Szebenyi K; Erdei Z; Varady G; Karaszi E; Homolya L; Nemet K; Gocza E; Miskey C; Mates L; Ivics Z; Izsvak Z; Sarkadi B
Applying a "double-feature" promoter to identify cardiomyocytes differentiated from human embryonic stem cells following transposon-based gene delivery
Stem Cells 27 (5): 1077-1087 (2009-02-20)
Staunstrup NH; Moldt B; Mates L; Villesen P; Jakobsen M; Ivics Z; Izsvak Z; Mikkelsen JG
Hybrid lentivirus-transposon vectors with a random integration profile in human cells
Molecular Therapy 17 (7): 1205-1214 (2009-07)
Mates L; Chuah MK; Belay E; Jerchow B; Manoj N; Acosta-Sanchez A; Grzela DP; Schmitt A; Becker K; Matrai J; Ma L; Samara-Kuko E; Gysemans C; Pryputniewicz D; Miskey C; Fletcher B; Vandendriessche T; Ivics Z; Izsvak Z
Molecular evolution of a novel hyperactive Sleeping Beauty transposase enables robust stable gene transfer in vertebrates
Nature Genetics 41 (6): 753-761 (2009-06)
2008
Sinzelle L; Kapitonov VV; Grzela DP; Jursch T; Jurka J; Izsvak Z; Ivics Z
Transposition of a reconstructed Harbinger element in human cells and functional homology with two transposon-derived cellular genes
Proceedings of the National Academy of Sciences of the United States of America 105 (12): 4715-4720 (2008-03-25)
Takeda J; Izsvak Z; Ivics Z
Insertional mutagenesis of the mouse germline with Sleeping Beauty transposition
Methods in Molecular Biology 435: 109-125 (2008)
Walisko O; Schorn A; Rolfs F; Devaraj A; Miskey C; Izsvak Z; Ivics Z
Transcriptional activities of the Sleeping Beauty transposon and shielding its genetic cargo with insulators
Molecular Therapy 16 (2): 359-369 (2008-02)
Voigt K; Izsvak Z; Ivics Z
Targeted gene insertion for molecular medicine
Journal of Molecular Medicine 86 (11): 1205-1219 (2008-11)
Aitman TJ; Critser JK; Cuppen E; Dominiczak A; Fernandez-Suarez XM; Flint J; Gauguier D; Geurts AM; Gould M; Harris PC; Holmdahl R; Huebner N; Izsvak Z; Jacob HJ; Kuramoto T; Kwitek AE; Marrone A; Mashimo T; Moreno C; Mullins J; Mullins L; Olsson T; Pravenec M; Riley L; Saar K; Serikawa T; Shull JD; Szpirer C; Twigger SN; Voigt B; Worley K
Progress and prospects in rat genetics: a community view
Nature Genetics 40 (5): 516-22 (2008-04-28)
2007
Miskey C; Papp B; Mates L; Sinzelle L; Keller H; Izsvak Z; Ivics Z
The Ancient Mariner Sails Again: Transposition of the Human Hsmar1 Element by a Reconstructed Transposase and Activities of the SETMAR Protein on Transposon Ends
Molecular and Cellular Biology 27 (12): 4589-4600 (2007-04-02)
Ivics Z; Katzer A; Stuewe EE; Fiedler D; Knespel S; Izsvak Z
Targeted Sleeping Beauty transposition in human cells
Molecular Therapy 15 (6): 1137-1144 (2007-06)
Mates L; Izsvak Z; Ivics Z
Technology transfer from worms and flies to vertebrates: transposition-based genome manipulations and their future perspectives
Genome Biology 8 Suppl 1: S1 (2007)
2006
Ivics Z; Izsvak Z
Transposons for gene therapy!
Current Gene Therapy 6 (5): 593-607 (2006-10)
Izsvak Z; Ivics Z; Mates L
Hungary's 'reforms' are threatening basic science
Nature 443 (7110): 394 (2006-09-28)
Ivics Z; Walisko O; Izsvak Z
Transposons
Encyclopedic Reference of Genomics and Proteomics in Molecular Medicine ; Vol. 2 : 1915-1919 (2006-07-05)
Walisko O; Izsvak Z; Szabo K; Kaufman CD; Herold S; Ivics Z
Sleeping Beauty transposase modulates cell-cycle progression through interaction with Miz-1
Proceedings of the National Academy of Sciences of the United States of America 103 (11): 4062-4067 (2006-03-14)
2005
Kaufman CD; Izsvak Z; Katzer A; Ivics Z
Frog Prince transposon-based RNAi vectors mediate efficient gene knockdown in human cells
Journal of RNAi and Gene Silencing 1 (2): 97-104 (2005-10)
Izsvak Z; Ivics Z
Sleeping Beauty hits them all: transposon-mediated saturation mutagenesis in the mouse germline
Nature Methods 2 (10): 735-736 (2005-10)
Miskey C; Izsvak Z; Kawakami K; Ivics Z
DNA transposons in vertebrate functional genomics
Cellular and Molecular Life Sciences 62 (6): 629-641 (2005-03)
Ivics Z; Izsvak Z
A whole lotta jumpin' goin' on: new transposon tools for vertebrate functional genomics
Trends in Genetics 21: 8-11 (2005-01-01)
2004
Izsvak Z; Ivics Z
Sleeping Beauty transposition: biology and applications for molecular therapy
Molecular Therapy 9: 147-156 (2004-02-01)
Izsvak Z; Stuewe EE; Fiedler D; Katzer A; Jeggo PA; Ivics Z
Healing the wounds inflicted by Sleeping Beauty transposition by double-strand break repair in mammalian somatic cells
Molecular Cell 13: 279-290 (2004-01-01)
Ivics Z; Kaufman CD; Zayed H; Miskey C; Walisko O; Izsvak Z
The Sleeping Beauty transposable element: evolution, regulation and genetic applications
Current Issues in Molecular Biology 6: 43-55 (2004-01-01)
Zayed H; Izsvak Z; Walisko O; Ivics Z
Development of hyperactive Sleeping Beauty transposon vectors by mutational analysis
Molecular Therapy 9: 292-304 (2004-02-01)
Ivics Z; Izsvak Z
Transposable elements for transgenesis and insertional mutagenesis in vertebrates: a contemporary review of experimental strategies
Mobile Genetic Elements: Protocols and Genomic Applications : 255-276 (2004-01-01)
2003
Zayed H; Izsvak Z; Khare D; Heinemann U; Ivics Z
The DNA-bending protein HMGB1 is a cellular cofactor of Sleeping Beauty transposition
Nucleic Acids Research 31 (9): 2313-2322 (2003-05-01)
Miskey C; Izsvak Z; Plasterk RH; Ivics Z
The Frog Prince: a reconstructed transposon from Rana pipiens with high transpositional activity in vertebrate cells
Nucleic Acids Research 31 (23): 6873-6881 (2003-12-01)
2002
Izsvak Z; Khare D; Behlke J; Heinemann U; Plasterk RH; Ivics Z
Involvement of a bifunctional, paired-like DNA-binding domain and a transpositional enhancer in Sleeping Beauty transposition
Journal of Biological Chemistry 277: 34581-34588 (2002-01-01)
Vigdal TJ; Kaufman CD; Izsvak Z; Voytas DF; Ivics Z
Common physical properties of DNA affecting target site selection of Sleeping Beauty and other Tc1/mariner transposable elements
Journal of Molecular Biology 323: 441-452 (2002-10-25)
1998
Luo G; Ivics Z; Izsvak Z; Bradley A
Chromosomal transposition of a Tc1/mariner-like element in mouse embryonic stem cells
Proceedings of the National Academy of Sciences of the United States of America 95 (18): 10769-10773 (1998-09-01)