Methods in Molecular Biology 1193: 117-126 (2014)

Kieback E.; Hilgenberg E.; Fillatreau S.

A method for the generation of TCR retrogenic mice

Retrogenic mice provide a unique system for rapidly analyzing the function of genes in the hematopoietic system. Here, we provide a detailed protocol for the production of retrogenic mice expressing genes coding for T cell receptor (TCR) for antigen. This technology should be easy to establish in any laboratory and should allow for a rapid progress in our understanding of the functional roles of TCR repertoires in immunity.

Keywords: Mice, Animals, Transfection, Transduction, Retrovirus, Retrogenic Mice, T Cell Receptor for Antigen (TCR)

Type: Article

PubMed: 25151002
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