In vivo lineage analysis of pancreatic and hepatic precursor cells
Pdx1 E9.5 mouse in situ
One main focus of our laboratory is to investigate how
pancreatic versus hepatic fate decision occurs in the
endoderm at both the cellular and molecular level.
To conduct a comprehensive in vivo analysis of the
hepatic-pancreatic lineage in the mouse embryo,
we use transgenic reporter models that express
fluorescent proteins under the control of lineage-specific
promoters. We are using these new genetic tools to:
i. address in vivo and in vitro if the liver and pancreas
arise from a common bipotent precursor; and
ii. trace and molecularly profile the presumptive
precursor cell and its descendants in the mouse embryo.
All together, these experiments will determine how the
hepatic-pancreatic lineage is established in vivo, whether
a bipotent endodermal precursor exists, and provide us
with its molecular signature.