We devised a method to collect staged C. elegans embryos by fluorescence-activated cell sorting (eFACS). A single eFACS run routinely yields tens of thousands of almost perfectly staged one-cell stage embryos.
In principle, eFACS can be used to extract large samples of embryos enriched in any desired embryonic stage. Thus, eFACS opens the door to apply many modern high-throughput technologies to assay embryonic developmental stage specific gene expression in C. elegans. Several of such investigations are already ongoing in the lab.