Publikationen



2017

Piwecka M.; Glazar P.; Hernandez-Miranda L.R.; Memczak S.; Wolf S.A.; Rybak-Wolf A.; Filipchyk A.; Klironomos F.; Cerda Jara C.A.; Fenske P.; Trimbuch T.; Zywitza V.; Plass M.; Schreyer L.; Ayoub S.; Kocks C.; Kuehn R.; Rosenmund C.; Birchmeier C.; Rajewsky N.
Loss of a mammalian circular RNA locus causes miRNA deregulation and affects brain function
Science 357 (6357): eaam8526 (2017-09-22)



Biagosch C.; Ediga R.D.; Hensler S.V.; Faerberboeck M.; Kuehn R.; Wurst W.; Meitinger T.; Koelker S.; Sauer S.; Prokisch H.
Elevated glutaric acid levels in Dhtkd1-/Gcdh- double knockout mice challenge our current understanding of lysine metabolism
Biochimica et Biophysica Acta - Molecular Basis of Disease 1863 (9): 2220-2228 (2017-09)



Yumlu S.; Stumm J.; Bashir S.; Dreyer A.K.; Lisowski P.; Danner E.; Kuehn R.
Gene editing and clonal isolation of human induced pluripotent stem cells using CRISPR/Cas9
Methods 121-122: 29-44 (2017-05-15)



Wefers B.; Bashir S.; Rossius J.; Kuehn R.
Gene editing in mouse zygotes using the CRISPR/Cas9 system
Methods 121-122: 55-67 (2017-05-15)



2016

Weber T.; Namikawa K.; Winter B.; Mueller-Brown K.; Kuehn R.; Wurst W.; Koester R.W.
Caspase-mediated apoptosis induction in zebrafish cerebellar Purkinje neurons
Development 143 (22): 4279-4287 (2016-11-15)



Chu V.T.; Graf R.; Wirtz T.; Weber T.; Favret J.; Li X.; Petsch K.; Tran N.T.; Sieweke M.H.; Berek C.; Kuehn R.; Rajewsky K.
Efficient CRISPR-mediated mutagenesis in primary immune cells using CrispRGold and a C57BL/6 Cas9 transgenic mouse line
Proceedings of the National Academy of Sciences of the United States of America 113 (44): 12514-12519 (2016-11-01)



2015

Garrett L.; Zhang J.; Zimprich A.; Niedermeier K.M.; Fuchs H.; Gailus-Durner V.; Hrabe de Angelis M.; Vogt Weisenhorn D.; Wurst W.; Hoelter S.M.
Conditional reduction of adult born doublecortin-positive neurons reversibly impairs selective behaviors
Frontiers in Behavioral Neuroscience 9: 302 (2015-11-12)



Kuehn R.; Chu V.T.
Pop in, pop out: a novel gene-targeting strategy for use with CRISPR-Cas9
Genome Biology 16 (1): 244 (2015-11-10)



Truong D.J.J.; Kuehner K.; Kuehn R.; Werfel S.; Engelhardt S.; Wurst W.; Ortiz O.
Development of an intein-mediated split-Cas9 system for gene therapy
Nucleic Acids Research 43 (13): 6450-6458 (2015-07-27)



Chu V.T.; Weber T.; Wefers B.; Wurst W.; Sander S.; Rajewsky K.; Kuehn R.
Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells
Nature Biotechnology 33 (5): 543-548 (2015-05)



2014

Kuehn R.; Wefers B.
Editing and investigating genomes with TALE and CRISPR/Cas systems: Genome engineering across species using TALENs
Methods 69 (1): 1 (2014-08-15)



Meier F.; Giesert F.; Delic S.; Faus-Kessler T.; Matheus F.; Simeone A.; Hoelter S.M.; Kuehn R.; Vogt-Weisenhorn D.M.; Wurst W.; Prakash N.
FGF/FGFR2 signaling regulates the generation and correct positioning of Bergmann glia cells in the developing mouse cerebellum
PLoS ONE 9 (7): e101124 (2014-07-01)



2013

Wefers B.; Panda S.K.; Ortiz O.; Brandl C.; Hensler S.; Hansen J.; Wurst W.; Kuehn R.
Generation of targeted mouse mutants by embryo microinjection of TALEN mRNA
Nature Protocols 8 (12): 2355-2379 (2013-12)



Kim J.; Badaloni A.; Willert T.; Zimber-Strobl U.; Kuehn R.; Wurst W.; Kieslinger M.
An RNAi-based approach to down-regulate a gene family in vivo
PLoS ONE 8 (11): e80312 (2013-11-12)



Panda S.K.; Wefers B.; Ortiz O.; Floss T.; Schmid B.; Haass C.; Wurst W.; Kuehn R.
Highly efficient targeted mutagenesis in mice using TALENs
Genetics 195 (3): 703-713 (2013-11)



Wefers B.; Meyer M.; Ortiz O.; Hrabe de Angelis M.; Hansen J.; Wurst W.; Kuehn R.
Direct production of mouse disease models by embryo microinjection of TALENs and oligodeoxynucleotides
Proceedings of the National Academy of Sciences of the United States of America 110 (10): 3782-3787 (2013-03-05)



Bolze F.; Rink N.; Brumm H.; Kuehn R.; Mocek S.; Schwarz A.E.; Kless C.; Biebermann H.; Wurst W.; Rozman J.; Klingenspor M.
Characterization of the melanocortin-4-receptor nonsense mutation W16X in vitro and in vivo
Pharmacogenomics Journal 13 (1): 80-93 (2013-02)



Merkl C.; Saalfrank A.; Riesen N.; Kuehn R.; Pertek A.; Eser S.; Hardt M.S.; Kind A.; Saur D.; Wurst W.; Iglesias A.; Schnieke A.
Efficient generation of rat induced pluripotent stem cells using a non-viral inducible vector
PLoS ONE 8 (1): e55170 (2013-01-31)



2012

Klymiuk I.; Kenner L.; Adler T.; Busch D.H.; Boersma A.; Irmler M.; Fridrich B.; Gailus-Durner V.; Fuchs H.; Leitner N.; Mueller M.; Kuehn R.; Schlederer M.; Treise I.; de Angelis M.H.; Beckers J.
In vivo functional requirement of the mouse Ifitm1 gene for germ cell development, interferon mediated immune response and somitogenesis
PLoS ONE 7 (10): e44609 (2012-10-24)



Lehmann F.M.; Feicht S.; Helm F.; Maurberger A.; Ladinig C.; Zimber-Strobl U.; Kuehn R.; Mautner J.; Gerbitz A.; Bornkamm G.W.
Humanized c-Myc mouse
PLoS ONE 7 (7): e42021 (2012-07-31)



Meyer M.; Ortiz O.; Hrabe de Angelis M.; Wurst W.; Kuehn R.
Modeling disease mutations by gene targeting in one-cell mouse embryos
Proceedings of the National Academy of Sciences of the United States of America 109 (24): 9354-9359 (2012-06-12)



Glasl L.; Kloos K.; Giesert F.; Roethig A.; Di Benedetto B.; Kuehn R.; Zhang J.; Hafen U.; Zerle J.; Hofmann A.; de Angelis M.H.; Winklhofer K.F.; Hoelter S.M.; Vogt Weisenhorn D.M.; Wurst W.
Pink1-deficiency in mice impairs gait, olfaction and serotonergic innervation of the olfactory bulb
Experimental Neurology 235 (1): 214-227 (2012-05)



Wefers B.; Hitz C.; Hoelter S.M.; Truembach D.; Hansen J.; Weber P.; Puetz B.; Deussing J.M.; de Angelis M.H.; Roenneberg T.; Zheng F.; Alzheimer C.; Silva A.; Wurst W.; Kuehn R.
MAPK signaling determines anxiety in the juvenile mouse brain but depression-like behavior in adults
PLoS ONE 7 (4): e35035 (2012-04-18)



Di Benedetto B.; Kuehn R.; Nothdurfter C.; Rein T.; Wurst W.; Rupprecht R.
N-desalkylquetiapine activates ERK1/2 to induce GDNF release in C6 glioma cells: a putative cellular mechanism for quetiapine as antidepressant
Neuropharmacology 62 (1): 209-216 (2012-01)



2011

Hampel F.; Ehrenberg S.; Hojer C.; Draeseke A.; Marschall-Schroeter G.; Kuehn R.; Mack B.; Gires O.; Vahl C.J.; Schmidt-Supprian M.; Strobl L.J.; Zimber-Strobl U.
CD19-independent instruction of murine marginal zone B-cell development by constitutive Notch2 signaling
Blood 118 (24): 6321-631 (2011-12-08)



2010

Meyer M.; de Angelis M.H.; Wurst W.; Kuehn R.
Gene targeting by homologous recombination in mouse zygotes mediated by zinc-finger nucleases
Proceedings of the National Academy of Sciences of the United States of America 107 (34): 15022-15026 (2010-08-24)



Cox B.J.; Vollmer M.; Tamplin O.; Lu M.; Biechele S.; Gertsenstein M.; van Campenhout C.; Floss T.; Kuehn R.; Wurst W.; Lickert H.; Rossant J.
Phenotypic annotation of the mouse X chromosome
Genome Research 20 (8): 1154-1164 (2010-08)



2009

Brown S.D.M.; Wurst W.; Kuehn R.; Hancock J.M.
The functional annotation of mammalian genomes: the challenge of phenotyping
Annual Review of Genetics 43: 305-333 (2009-12)



2008

Delic S.; Streif S.; Deussing J.M.; Weber P.; Ueffing M.; Hoelter S.M.; Wurst W.; Kuehn R.
Genetic mouse models for behavioral analysis through transgenic RNAi technology
Genes Brain and Behavior 7 (7): 821-830 (2008-10)



2007

Hitz C.; Wurst W.; Kuehn R.
Conditional brain-specific knockdown of MAPK using Cre/loxP regulated RNA interference
Nucleic Acids Research 35 (12): e90 (2007-06)



Di Benedetto B.; Hitz C.; Hoelter S.M.; Kuehn R.; Vogt Weisenhorn D.M.; Wurst W.
Differential mRNA distribution of components of the ERK/MAPK signalling cascade in the adult mouse brain
Journal of Comparative Neurology 500 (3): 542-556 (2007-01-20)



Brenz Verca M.S.; Weber P.; Mayer C.; Graf C.; Refojo D.; Kuehn R.; Grummt I.; Lutz B.
Development of a species-specific RNA polymerase I-based shRNA expression vector
Nucleic Acids Research 35 (2): e10 (2007-01)



2006

Mori T.; Tanaka K.; Buffo A.; Wurst W.; Kuehn R.; Goetz M.
Inducible gene deletion in astroglia and radial glia--a valuable tool for functional and lineage analysis
Glia 54 (1): 21-34 (2006-07)



Chen A.P.; Ohno M.; Giese K.P.; Kuehn R.; Chen R.L.; Silva A.J.
Forebrain-specific knockout of B-raf kinase leads to deficits in hippocampal long-term potentiation, learning, and memory
Journal of Neuroscience Research 83 (1): 28-38 (2006-01)



2005

Seibler J.; Kueter-Luks B.; Kern H.; Streu S.; Plum L.; Mauer J.; Kuehn R.; Bruening J.C.; Schwenk F.
Single copy shRNA configuration for ubiquitous gene knockdown in mice
Nucleic Acids Research 33 (7): e67 (2005-04-14)



2003

Mueller M.B.; Zimmermann S.; Sillaber I.; Hagemeyer T.P.; Deussing J.M.; Timpl P.; Kormann M.S.D.; Droste S.K.; Kuehn R.; Reul J.M.H.M.; Holsboer F.; Wurst W.
Limbic corticotropin-releasing hormone receptor 1 mediates anxiety-related behavior and hormonal adaptation to stress
Nature Neuroscience 6 (10): 1100-1107 (2003-10)



Theis M.; Soehl G.; Speidel D.; Kuehn R.; Willecke K.
Connexin43 is not expressed in principal cells of mouse cortex and hippocampus
European Journal of Neuroscience 18 (2): 267-274 (2003-07)



Schwenk F.; Zevnik B.; Bruening J.; Roehl M.; Willuweit A.; Rode A.; Hennek T.; Kauselmann G.; Jaenisch R.; Kuehn R.
Hybrid embryonic stem cell-derived tetraploid mice show apparently normal morphological, physiological, and neurological characteristics
Molecular and Cellular Biology 23 (11): 3982-3989 (2003-06)



Seibler J.; Zevnik B.; Kueter-Luks B.; Andreas S.; Kern H.; Hennek T.; Rode A.; Heimann C.; Faust N.; Kauselmann G.; Schoor M.; Jaenisch R.; Rajewsky K.; Kuehn R.; Schwenk F.
Rapid generation of inducible mouse mutants
Nucleic Acids Research 31 (4): e12 (2003-02-15)



2002

Andreas S.; Schwenk F.; Kueter-Luks B.; Faust N.; Kuehn R.
Enhanced efficiency through nuclear localization signal fusion on phage PhiC31-integrase: activity comparison with Cre and FLPe recombinase in mammalian cells
Nucleic Acids Research 30 (11): 2299-2306 (2002-06-01)



Eggan K.; Rode A.; Jentsch I.; Samuel C.; Hennek T.; Tintrup H.; Zevnik B.; Erwin J.; Loring J.; Jackson-Grusby L.; Speicher M.R.; Kuehn R.; Jaenisch R.
Male and female mice derived from the same embryonic stem cell clone by tetraploid embryo complementation
Nature Biotechnology 20 (5): 455-459 (2002-05)



2001

Rios M.; Fan G.; Fekete C.; Kelly J.; Bates B.; Kuehn R.; Lechan R.M.; Jaenisch R.
Conditional deletion of brain-derived neurotrophic factor in the postnatal brain leads to obesity and hyperactivity
Molecular Endocrinology 15 (10): 1748-1757 (2001-10)



Lommel S.; Benesch S.; Rottner K.; Franz T.; Wehland J.; Kuehn R.
Actin pedestal formation by enteropathogenic Escherichia coli and intracellular motility of Shigella flexneri are abolished in N-WASP-defective cells
EMBO Reports 2 (9): 850-857 (2001-09)



Roberds S.L.; Anderson J.; Basi G.; Bienkowski M.J.; Branstetter D.G.; Chen K.S.; Freedman S.B.; Frigon N.L.; Games D.; Hu K.; Johnson-Wood K.; Kappenman K.E.; Kawabe T.T.; Kola I.; Kuehn R.; Lee M.; Liu W.; Motter R.; Nichols N.F.; Power M.; Robertson D.W.; Schenk D.; Schoor M.; Shopp GM.; Shuck M.E.; Sinha S.; Svensson K.A.; Tatsuno G.; Tintrup H.; Wijsman J.; Wright S.; McConlogue L.
BACE knockout mice are healthy despite lacking the primary beta-secretase activity in brain: implications for Alzheimer's disease therapeutics
Human Molecular Genetics 10 (12): 1317-1324 (2001-06-01)



Fan G.; Beard C.; Chen R.Z.; Csankovszki G.; Sun Y.; Siniaia M.; Biniszkiewicz D.; Bates B.; Lee P.P.; Kuehn R.; Trumpp A.; Poon C.; Wilson C.B.; Jaenisch R.
DNA hypomethylation perturbs the function and survival of CNS neurons in postnatal animals
Journal of Neuroscience 21 (3): 788-797 (2001-02-01)



1999

Minichiello L.; Korte M.; Wolfer D.; Kuehn R.; Unsicker K.; Cestari V.; Rossi-Arnaud C.; Lipp H.P.; Bonhoeffer T.; Klein R.
Essential role for TrkB receptors in hippocampus-mediated learning
Neuron 24 (2): 401-414 (1999-10)



1998

Schmedt C.; Saijo K.; Niidome T.; Kuehn R.; Aizawa S.; Tarakhovsky A.
Csk controls antigen receptor-mediated development and selection of T-lineage cells
Nature 394 (6696): 901-904 (1998-08-27)



Wynn T.A.; Cheever A.W.; Williams M.E.; Hieny S.; Caspar P.; Kuehn R.; Mueller W.; Sher A.
IL-10 regulates liver pathology in acute murine Schistosomiasis mansoni but is not required for immune down-modulation of chronic disease
Journal of Immunology 160 (9): 4473-4480 (1998-05-01)



Schwenk F.; Kuehn R.; Angrand P.O.; Rajewsky K.; Stewart A.F.
Temporally and spatially regulated somatic mutagenesis in mice
Nucleic Acids Research 26 (6): 1427-1432 (1998-03-15)



1997

Lam K.P.; Kuehn R.; Rajewsky K.
In vivo ablation of surface immunoglobulin on mature B cells by inducible gene targeting results in rapid cell death
Cell 90 (6): 1073-1083 (1997-09-19)



Kuehn R.; Schwenk F.
Advances in gene targeting methods
Current Opinion in Immunology 9 (2): 183-188 (1997-04)



1996

Morawetz R.A.; Gabriele L.; Rizzo L.V.; Noben-Trauth N.; Kuehn R.; Rajewsky K.; Mueller W.; Doherty T.M.; Finkelman F.; Coffman R.L.; Morse H.C.
Interleukin (IL)-4-independent immunoglobulin class switch to immunoglobulin (Ig)E in the mouse
Journal of Experimental Medicine 184 (5): 1651-1661 (1996-11-01)



Beissert S.; Hosoi J.; Kuehn R.; Rajewsky K.; Mueller W.; Granstein R.D.
Impaired immunosuppressive response to ultraviolet radiation in interleukin-10-deficient mice
Journal of Investigative Dermatology 107 (4): 553-557 (1996-10)



Berg D.J.; Davidson N.; Kuehn R.; Mueller W.; Menon S.; Holland G.; Thompson-Snipes L.; Leach M.W.; Rennick D.
Enterocolitis and colon cancer in interleukin-10-deficient mice are associated with aberrant cytokine production and CD4(+) TH1-like responses
Journal of Clinical Investigation 98 (4): 1010-1020 (1996-08-15)



Texido G.; Jacobs H.; Meiering M.; Kuehn R.; Roes J.; Mueller W.; Gilfillan S.; Fujiwara H.; Kikutani H.; Yoshida N.; Amakawa R.; Benoist C.; Mathis D.; Kishimoto T.; Mak T.W.; Rajewsky K.
Somatic hypermutation occurs in B cells of terminal deoxynucleotidyl transferase-, CD23-, interleukin-4-, IgD- and CD30-deficient mouse mutants
European Journal of Immunology 26 (8): 1966-1969 (1996-08)



Rajewsky K.; Gu H.; Kuehn R.; Betz U.A.; Mueller W.; Roes J.; Schwenk F.
Conditional gene targeting
Journal of Clinical Investigation 98 (3): 600-603 (1996-08-01)



Gazzinelli R.T.; Wysocka M.; Hieny S.; Scharton-Kersten T.; Cheever A.; Kuehn R.; Mueller W.; Trinchieri G.; Sher A.
In the absence of endogenous IL-10, mice acutely infected with Toxoplasma gondii succumb to a lethal immune response dependent on CD4+ T cells and accompanied by overproduction of IL-12, IFN-gamma and TNF-alpha
Journal of Immunology 157 (2): 798-805 (1996-07-15)



Davidson N.J.; Leach M.W.; Fort M.M.; Thompson-Snipes L.; Kuehn R.; Mueller W.; Berg D.J.; Rennick D.M.
T helper cell 1-type CD4+ T cells, but not B cells, mediate colitis in interleukin 10-deficient mice
Journal of Experimental Medicine 184 (1): 241-251 (1996-07-01)



Carrera L.; Gazzinelli R.T.; Badolato R.; Hieny S.; Mueller W.; Kuehn R.; Sacks D.L.
Leishmania promastigotes selectively inhibit interleukin 12 induction in bone marrow-derived macrophages from susceptible and resistant mice
Journal of Experimental Medicine 183 (2): 515-526 (1996-02-01)



Sobol R.W.; Horton J.K.; Kuehn R.; Gu H.; Singhal R.K.; Prasad R.; Rajewsky K.; Wilson S.H.
Requirement of mammalian DNA polymerase-beta in base-excision repair
Nature 379 (6561): 183-186 (1996-01-11)



1995

Di Santo J.P.; Kuehn R.; Mueller W.
Common cytokine receptor gamma chain (gamma c)-dependent cytokines: understanding in vivo functions by gene targeting
Immunological Reviews 148 (1): 19-34 (1995-12)



Berg D.J.; Kuehn R.; Rajewsky K.; Mueller W.; Menon S.; Davidson N.; Gruenig G.; Rennick D.
Interleukin-10 is a central regulator of the response to LPS in murine models of endotoxic shock and the Shwartzman reaction but not endotoxin tolerance
Journal of Clinical Investigation 96 (5): 2339-2347 (1995-11)



Kuehn R.; Schwenk F.; Aguet M.; Rajewsky K.
Inducible gene targeting in mice
Science 269 (5229): 1427-1429 (1995-09-08)



Bachmann M.F.; Schorle H.; Kuehn R.; Mueller W.; Hengartner H.; Zinkernagel R.M.; Horak I.
Antiviral immune responses in mice deficient for both interleukin-2 and interleukin-4
Journal of Virology 69 (8): 4842-4846 (1995-08)



Berg D.J.; Leach M.W.; Kuehn R.; Rajewsky K.; Mueller W.; Davidson N.J.; Rennick D.
Interleukin 10 but not interleukin 4 is a natural suppressant of cutaneous inflammatory responses
Journal of Experimental Medicine 182 (1): 99-108 (1995-07-01)



1994

Schmitt E.; Germann T.; Goedert S.; Hoehn P.; Huels C.; Koelsch S.; Kuehn R.; Mueller W.; Palm N.; Ruede E.
IL-9 production of naive CD4+ T cells depends on IL-2, is synergistically enhanced by a combination of TGF-beta and IL-4, and is inhibited by IFN-gamma
Journal of Immunology 153 (9): 3989-3996 (1994-11-01)



Fehling H.J.; Swat W.; Laplace C.; Kuehn R.; Rajewsky K.; Mueller U.; von Boehmer H.
MHC class I expression in mice lacking the proteasome subunit LMP-7
Science 265 (5176): 1234-1237 (1994-08-26)



Morawetz R.A.; Doherty T.M.; Giese N.A.; Hartley J.W.; Mueller W.; Kuehn R.; Rajewsky K.; Coffman R.; Morse H.C.
Resistance to murine acquired immunodeficiency syndrome (MAIDS)
Science 265 (5169): 264-266 (1994-07-08)



Schmitz J.; Thiel A.; Kuehn R.; Rajewsky K.; Mueller W.; Assenmacher M.; Radbruch A.
Induction of interleukin 4 (IL-4) expression in T helper (Th) cells is not dependent on IL-4 from non-Th cells
Journal of Experimental Medicine 179 (4): 1349-53 (1994-04-01)



Sadlack B.; Kuehn R.; Schorle H.; Rajewsky K.; Mueller W.; Horak I.
Development and proliferation of lymphocytes in mice deficient for both interleukins-2 and -4
European Journal of Immunology 24 (1): 281-284 (1994-01)



1993

Kuehn R.; Loehler J.; Rennick D.; Rajewsky K.; Mueller W.
Interleukin-10-deficient mice develop chronic enterocolitis
Cell 75 (2): 263-274 (1993-10-22)



Leal L.M.C.C.; Moss D.W.; Kuhn R.; Mueller W.; Liew F.Y.
Interleukin-4 transgenic mice of resistant background are susceptible to Leishmania major infection
European Journal of Immunology 23 (2): 566-569 (1993-02)



1992

Schultz C.L.; Rothman P.; Kuehn R.; Kehry M.; Mueller W.; Rajewsky K.; Alt F.; Coffman R.L.
T helper cell membranes promote IL-4-independent expression of germ-line C {gamma} 1 transcripts in B cells
Journal of Immunology 149 (1): 60-64 (1992-07-01)



1991

Kuehn R.; Rajewsky K.; Mueller W.
Generation and analysis of interleukin-4 deficient mice
Science 254 (5032): 707-710 (1991-11-01)



Kitamura D.; Roes J.; Kuehn R.; Rajewsky K.
A B cell-deficient mouse by targeted disruption of the membrane exon of the immunoglobulin mu chain gene
Nature 350 (6317): 423-426 (1991-04-04)



Mueller W.; Kuehn R.; Rajewsky K.
Major histocompatibility complex class II hyperexpression on B cells in interleukin 4-transgenic mice does not lead to B cell proliferation and hypergammaglobulinemia
European Journal of Immunology 21 (4): 921-925 (1991-04)



1985

Mueller W.; Kuehn R.; Goldmann W.; Tesch H.; Smith F.I.; Radbruch A.; Rajewsky K.
Signal requirements for growth and differentiation of activated murine B lymphocytes
Journal of Immunology 135 (2): 1213-1219 (1985-08)