Characterization of transcription termination-associated RNAs: new insights into their biogenesis, tailing, and expression in primary tumors

Autor/innen

  • I. Laudadio
  • S. Formichetti
  • S. Gioiosa
  • F. Klironomos
  • N. Rajewsky
  • G. Macino
  • C. Carissimi
  • V. Fulci

Journal

  • International Journal of Genomics

Quellenangabe

  • Int J Genomics 2018: 1243858

Zusammenfassung

  • Next-generation sequencing has uncovered novel classes of small RNAs (sRNAs) in eukaryotes, in addition to the well-known miRNAs, siRNAs, and piRNAs. In particular, sRNA species arise from transcription start sites (TSSs) and the transcription termination sites (TTSs) of genes. However, a detailed characterization of these new classes of sRNAs is still lacking. Here, we present a comprehensive study of sRNAs derived from TTSs of expressed genes (TTSa-RNAs) in human cell lines and primary tissues. Taking advantage of sRNA-sequencing, we show that TTSa-RNAs are present in the nuclei of human cells, are loaded onto both AGO1 and AGO2, and their biogenesis does not require DICER and AGO2 endonucleolytic activity. TTSa-RNAs display a strong bias against a G residue in the first position at 5 end, a known feature of AGO-bound sRNAs, and a peculiar oligoA tail at 3 end. AGO-bound TTSa-RNAs derive from genes involved in cell cycle progression regulation and DNA integrity checkpoints. Finally, we provide evidence that TTSa-RNAs can be detected by sRNA-Seq in primary human tissue, and their expression increases in tumor samples as compared to nontumor tissues, suggesting that in the future, TTSa-RNAs might be explored as biomarker for diagnosis or prognosis of human malignancies.


DOI

doi:10.1155/2018/1243858