Generating knockout rats by transposon mutagenesis in spermatogonial stem cells

Autor/innen

  • Z. Izsvak
  • J. Froehlich
  • I. Grabundzija
  • J.R. Shirley
  • H.M. Powell
  • K.M. Chapman
  • Z. Ivics
  • F.K. Hamra

Journal

  • Nature Methods

Quellenangabe

  • Nat Methods 7 (6): 443-445

Zusammenfassung

  • Disrupting genes in the rat on a genome-wide scale will allow the investigation of many biological processes linked to human health. Here we used transposon-mediated mutagenesis to knock out genes in rat spermatogonial stem cells. Given the capacity of the testis to support spermatogenesis from thousands of transplanted, genetically manipulated spermatogonia, this approach paves a way for high-throughput functional genomic studies in the laboratory rat.


DOI

doi:10.1038/nmeth.1461