Sometimes a lack of oxygen in the kidneys leads to renal failure – as seen in some heart conditions, or in the aftermath of massive bleeding or treatment with particular drugs. The team of scientists have now used in-vivo imaging techniques to show that a cellular signaling protein called NF-κB becomes activated inappropriately in the kidneys after ischemia. NF-κB is a transcription factor: a molecule that activates genes, and in other tissues those genes are associated with functions such as programmed cell death, inflammations and immune responses. Because it has so many important tasks in the body, simply targeting the protein with drugs is usually not an option for treatment.
But the scientists’ work has now brought the molecule back into play. “We developed a unique mouse model in which we deactivated the NF-κB molecule very specifically in renal tubular epithelial cells,” says Dr Markó. Following an artificially induced case of ischemia, these mice experienced considerably less tissue damage and necrosis, and had far fewer sites of inflammation than control animals. The loss of NF-κB reduced the activity of its normal target genes in the kidneys. And in laboratory cultures of tubular cells, suppressing the signaling pathway allowed more cells to survive while reducing the release of inflammatory factors./p>
The scientists hope that tracing ischemia’s effects to NF-κB activity in a specific type of cell might be a first step toward developing a future therapy tailor-made to target it. And the strain of mouse developed for the project can be used to study the activity of other proteins in tubular cells, providing a way to study other kidney diseases that affect them.
The findings emerged from a cooperation between the working groups of Prof. Dominik N. Müller (ECRC), Dr. Ruth Schmidt-Ulrich (MDC), and Prof. Kai Schmidt-Ott (MDC, Charité), which was part of a project funded by the German Research Foundation (Research Unit 1368).
Lajos Markó, Emilia Vigolo, Christian Hinze, Joon-Keun Park, Giulietta Roël, András Balogh,Mira Choi, Anne Wübken, Jimmi Cording, Ingolf E. Blasig, Friedrich C. Luft, Claus Scheidereit, Kai M. Schmidt-Ott, Ruth Schmidt-Ullrich, und Dominik N. Müller (2015): “Tubular Epithelial NF-kB Activity Regulates Ischemic AKI.” Journal of the American Society of Nephrology 27. DOI: 10.1681/ASN.2015070748
Experimental and Clinical Research Center (ECRC), a joint institution of the Charité Universitätsmedizin Berlin and the Max-Delbrück Center; Max-Delbrück Center for Molecular Medicine; Hannover Medical School (MHH); Leibniz-Institut für molekulare Pharmakologie (FMP); 5Medical Department, Division of Nephrology, Charité, Berlin
Lajos Markó and Emilia Vigolo contributed equally to this work. Kai M. Schmidt-Ott, Ruth Schmidt-Ullrich and Dominik N. Müller contributed equally as principal investigators.