Mildner Zentrifuge

New therapeutic options for multiple sclerosis in sight

Strategies for treating multiple sclerosis have so far focused primarily on T and B cells. A group of MDC researchers has now unveiled a new approach in Nature Immunology – one that seeks to increase treatment effectiveness by selectively targeting another type of immune cell called monocytes.

Multiple sclerosis (MS) is known as “the disease with a thousand faces” because symptoms and progression can vary dramatically from patient to patient. But every MS patient has one thing in common: Cells of their body’s own immune system migrate to the brain, where they destroy the myelin sheath – the protective outer layer of the nerve fibers. As a result, an electrical short circuit occurs, which prevents the nerve signals from being transmitted properly.

Many MS medications impair immune memory

Researchers don’t yet know exactly which immune cells are involved in stripping away the myelin sheath. Autoreactive T and B cells, which wrongly identify the myelin sheath as a foreign body, travel to the brain and initiate the disease. “Up until now, MS drugs have essentially targeted these T and B cells, both of which are part of the acquired immune system,” says Dr. Alexander Mildner, a scientist at the Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC) and the senior author of the paper now published in Nature Immunology.

Alexander Mildner separates monocytes originally from the central nervous system from myelin by centrifugation.

Mildner is currently conducting externally funded research as a DFG Heisenberg fellow in Professor Achim Leutz’s lab at the MDC, which focuses on cell differentiation and tumorigenesis. “But by attacking the acquired immune system, the MS drugs adversely affect the body’s immune memory, thus making patients more susceptible to infections in the long run,” the scientist says.

MS symptoms improved in mice by reducing monocytes

As a result, Mildner has been pursuing a different strategy for a couple of years now. He wants to find out what role immune cells – particularly those that are part of innate immunity – play in the development of MS and whether they represent a promising target structure for therapy of MS patients. “In an earlier study with a mouse model of MS, we were able to show that disease symptoms in the mice declined significantly within a few days after their monocytes were selectively destroyed by antibodies,” the researcher reports. This result came as a big surprise to him and to many of his colleagues. “Apparently, it is not only T and B cells that are involved in causing tissue damage in MS,” Mildner says.

The monocytes he studied are a special type of white blood cells that shortly circulate in the blood before migrating into tissue. Once there, they transform themselves into effector cells (phagocytes) and destroy foreign tissue in the central nervous system (CNS) – or which, during MS, they wrongly identify as such. “This process,” Mildner says, “leads to inflammation and tissue damage in the brain.”

The team discovered unknown types of monocytes

For the subsequent transcriptome analysis Alexander Mildner controls sorted monocytes under the microscope.

In the current study published in Nature Immunology, which he conducted in collaboration with an Israeli team led by Professor Ido Amit from the Department of Immunology at the Weizmann Institute of Science, Mildner and his team also focused on monocytes. “During the last recent years we realized that several types of these immune cells exits, which might carry out different functions,” the researcher says. “We therefore wanted to examine in our mouse model of MS the monocytes in greater detail using single-cell sequencing and to find out, which monocyte subsets are present in the brain in MS and are responsible for tissue damage.”

He and his colleagues identified six different monocyte subtypes, four of which were previously unknown. As in his earlier study, Mildner injected the mice with antibodies against a specific monocyte surface protein. As expected, the cells died and the MS symptoms in the mice decreased within a short period of time. “But what surprised us was that the antibodies did not destroy all monocyte subsets in the brain that have this surface protein,” Mildner says.

Not all monocytes destroy the protective myelin sheath

Single cell analysis – Each colour represents a group of monocytes with similar transcriptomes.

“Only a certain type of monocyte, the Cxcl10+ cells, was destroyed by the antibody treatment,” Mildner says. “These are apparently the cells that are primarily responsible for causing MS tissue damage in the brain.” With the help of single-cell sequencing, he and his team also discovered that this cell type differs from other monocytes in two essential ways: First, Cxcl10+ cells have a particularly large number of receptors for a signal protein secreted by T cells that induces tissue damaging properties in monocytes. Second, these cells produce large amounts of interleukin-1-beta, a substance that opens the blood-brain barrier, enabling immune cells to more easily pass from the blood to the brain and exacerbate the symptoms. “Our research suggests that T cells, as disease initiators, travel to the CNS in order to lure there the monocytes that are responsible for the primary tissue damage,” Mildner explains.

The other monocyte subsets that were identified, he speculates, are perhaps even involved in repair processes in which the body tries to rebuild the damaged myelin. In light of the study’s findings, he thinks it is also possible that the T and B cells are not even directly involved in stripping away the myelin sheath, but only indirectly in that they prompt the Cxcl10+ monocytes to attack the protective layer of the axons.

Many side effects may be preventable

“If that is the case, in the future most forms of MS could be treated by specifically deactivating the Cxcl10+ monocytes instead of targeting the T or B cells of the immune system,” Mildner says. “This would protect the body’s immune memory and prevent many side effects of current MS therapies.” The researcher and his team next plan to investigate whether the Cxcl10+ monocytes are also present outside the CNS. “If they exist in the body’s periphery, for example, in the lymph nodes,” he says, “there they would be easier to target with therapeutics than in the brain.”

Text: Anke Brodmerkel
 

Further information

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  • Alexander Mildner separates monocytes originally from the central nervous system from myelin by centrifugation. Foto: Alexander Mildner, MDC
  • For the subsequent transcriptome analysis Alexander Mildner controls sorted monocytes under the microscope. Foto: Alexander Mildner, MDC
  • Single cell analysis – Each colour represents a group of monocytes with similar transcriptomes Foto: Alexander Mildner, MDC

Literature

Amir Giladi et al. (2020):  „Cxcl10+ monocytes define a pathogenic subset in the central nervous system during autoimmune neuroinflammation”, Nature Immunology, DOI: 10.1038/s41590-020-0661-1

Contacts

Dr. Alexander Mildner
Head of an independent research group at the MDC (affiliated to the research group "Cell Differentiation and Tumorigenesis" of Professor Achim Leutz)
Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC)

Christina Anders
Editor, Communications Department
Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC)
+49 (0)30 9406 2118
christina.anders@mdc-berlin.de or presse@mdc-berlin.de

The Max Delbrück Center for Molecular Medicine (MDC)

 

The Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC) was founded in Berlin in 1992. It is named for the German-American physicist Max Delbrück, who was awarded the 1969 Nobel Prize in Physiology and Medicine. The MDC's mission is to study molecular mechanisms in order to understand the origins of disease and thus be able to diagnose, prevent and fight it better and more effectively. In these efforts the MDC cooperates with the Charité - Universitätsmedizin Berlin and the Berlin Institute of Health (BIH ) as well as with national partners such as the German Center for Cardiovascular Research and numerous international research institutions. More than 1,600 staff and guests from nearly 60 countries work at the MDC, just under 1,300 of them in scientific research. The MDC is funded by the German Federal Ministry of Education and Research (90 percent) and the State of Berlin (10 percent), and is a member of the Helmholtz Association of German Research Centers. www.mdc-berlin.de