The LUMIER assay, introduced by Barrios-Rodiles et al. in 2005, is a method for luminescence-based mammalian interactome mapping. The method is not radically different from other interaction assays but allows comparative measurements and has superior sensitivity and scalability.
We use LUMIER to validate PPIs detected in the Y2H screenings. For this purpose, we established an improved, robotics-based version of the LUMIER assay that, in contrast to conventional co-immunoprecipitation and Western blotting procedures, permits high-throughput experimentation.