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Adipocyte plasma membranes contain two Gi subtypes but are devoid of Go

Authors

  • K.D. Hinsch
  • W. Rosenthal
  • K. Spicher
  • T. Binder
  • H. Gausepohl
  • R. Frank
  • G. Schultz
  • H.G. Joost

Journal

  • FEBS Letters

Citation

  • FEBS Lett 238 (1): 191-196

Abstract

  • Antisera generated against synthetic peptides were used to identify G-protein alpha-subunits in plasma membranes from rat adipocytes. Applying the immunoblot technique, we detected two Gs alpha-subunits of 42 and 43 kDa, corresponding to the two cholera toxin substrates, and two Gi alpha-subunits of 40 and 41 kDa, corresponding to the two pertussis toxin substrates present in these membranes. The 40 kDa protein was tentatively identified as the Gi2 alpha-subunit. A serum specific for the Go alpha-subunit failed to detect any immunoreactive protein. Thus plasma membranes of adipocytes possess two forms of Gi but not Go.


DOI

doi:10.1016/0014-5793(88)80254-0