folder

Allogeneic vaccination for renal cell carcinoma: development and monitoring

Authors

  • H. Pohla
  • B. Frankenberger
  • B. Stadlbauer
  • R. Oberneder
  • A. Hofstetter
  • G. Willimsky
  • A. Pezzutto
  • B. Doerken
  • T. Blankenstein
  • D.J. Schendel

Journal

  • Bone Marrow Transplantation

Citation

  • Bone Marrow Transplant 25 Suppl. 2 (3332): S83-S87

Abstract

  • An allogeneic tumor cell vaccine should display a natural immunogenicity that allows the stimulation of tumor-reactive effector cells in patients. Furthermore, the vaccine should express antigens that are shared by many tumors to which patients are not tolerant. A variety of tumor peptides should be presented by different HLA-molecules due to limited MHC matching with recipients and last but not least, the vaccine should have a strong growth potential in vitro to allow adequate amounts of vaccine to be generated for long-term usage. In vitro and in situ studies with the renal cell carcinoma cell line RCC-26 demonstrate: (1) RCC-26 can induce complex allospecific responses through direct priming; (2) RCC-26 can not only reactivate cytotoxic T lymphocytes (CTL) of a memory phenotype but they also can induce de novo tumor-antigen associated responses in normal donors; (3) these cells present epitopes restricted by several MHC molecules, allowing the vaccination of patients matched for different HLA alleles; and (4) they stimulate HLA-A*0201-restricted T cells bearing characteristic T cell receptors (TCR). Thus, in addition to using limiting dilution killer and ELISPOT assays, molecular tracking of a tumor-specific TCR can be used to judge the development of antitumor reactivity and vaccine efficiency.


DOI

doi:bmt/journal/v25/n2s/abs/1702362a.html