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Cooperation between C/EBP alphaTBP/TFIIB and SWI/SNF recruiting domains is required for adipocyte differentiation

Authors

  • T.A. Pedersen
  • E. Kowenz-Leutz
  • A. Leutz
  • C. Nerlov

Journal

  • Genes & Development

Citation

  • Genes Dev 15 (21): 3208-3216

Abstract

  • Chromatin remodeling is an important step in promoter activation during cellular lineage commitment and differentiation. We show that the ability of the C/EBPα transcription factor to direct adipocyte differentiation of uncommitted fibroblast precursors and to activate SWI/SNF-dependent myeloid-specific genes depends on a domain, C/EBP{alpha} transactivation element III (TE-III), that binds the SWI/SNF chromatin remodeling complex. TE-III collaborates with C/EBP{alpha} TBP/TFIIB interaction motifs during induction of adipogenesis and adipocyte-specific gene expression. These results indicate that C/EBP{alpha} acts as a lineage-instructive transcription factor through SWI/SNF-dependent modification of the chromatin structure of lineage-specific genes, followed by direct promoter activation via recruitment of the basal transcription-initiation complex, and provide a mechanism by which C/EBPα can mediate differentiation along multiple cellular lineages.