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Cytoskeletal components define protein location to membrane microdomains

Authors

  • W.G. Szymanski
  • H. Zauber
  • A. Erban
  • X.N. Wu
  • W.X. Schulze

Journal

  • Molecular & Cellular Proteomics

Citation

  • Mol Cell Proteomics 14 (9): 2493-2509

Abstract

  • The plasma membrane is an important compartment undergoing dynamic changes in composition upon external or internal stimuli. The dynamic subcompartmentation of proteins in ordered low density (DRM) and disordered high density (DSM) membrane phases is hypothesized to require interactions with cytoskeletal components. Here we systematically analyzed the effects of actin or tubulin disruption on the distribution of proteins between membrane density phases. We used a proteomic screen to identify candidate proteins with altered submembrane location followed by biochemical or cell biological characterization in Arabidopsis thaliana. We found that several proteins, such as plasma membrane ATPases, receptor kinases or remorins resulted in a differential distribution between membrane density phases upon cytoskeletal disruption. Moreover, in most cases contrasting effects were observed: Disruption of actin filaments largely lead to a redistribution of proteins from DRM to DSM membrane fractions while disruption of tubulins resulted in general depletion of proteins from the membranes. We conclude that actin filaments are necessary for dynamic movement of proteins between different membrane phases and that microtubules are not necessarily important for formation of microdomains as such, but rather they may control the protein amount present in the membrane phases.


DOI

doi:10.1074/mcp.M114.046904