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Efficient Bayesian-based multiview deconvolution

Authors

  • S. Preibisch
  • F. Amat
  • E. Stamataki
  • M. Sarov
  • R.H. Singer
  • E. Myers
  • P. Tomancak

Journal

  • Nature Methods

Citation

  • Nat Methods 11 (6): 645-648

Abstract

  • Light-sheet fluorescence microscopy is able to image large specimens with high resolution by capturing the samples from multiple angles. Multiview deconvolution can substantially improve the resolution and contrast of the images, but its application has been limited owing to the large size of the data sets. Here we present a Bayesian-based derivation of multiview deconvolution that drastically improves the convergence time, and we provide a fast implementation using graphics hardware.


DOI

doi:10.1038/nmeth.2929