folder

Endogenous MHC class II processing of a viral nuclear antigen after autophagy

Authors

  • C. Paludan
  • D. Schmid
  • M. Landthaler
  • M. Vockerodt
  • D. Kube
  • T. Tuschl
  • C. Muenz

Journal

  • Science

Citation

  • Science 307 (5709): 593-596

Abstract

  • CD4+ T cells classically recognize antigens that are endocytosed and processed in lysosomes for presentation on major histocompatibility complex (MHC) class II molecules. Here, endogenous Epstein-Barr virus nuclear antigen 1 (EBNA1) was found to gain access to this pathway by autophagy. On inhibition of lysosomal acidification, EBNA1, the dominant CD4+ T cell antigen of latent Epstein-Barr virus infection, slowly accumulated in cytosolic autophagosomes. In addition, inhibition of autophagy decreased recognition by EBNA1-specific CD4+ T cell clones. Thus, lysosomal processing after autophagy may contribute to MHC class II-restricted surveillance of long-lived endogenous antigens including nuclear proteins relevant to disease.


DOI

doi:10.1126/science.1104904