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Experimental applications of TNF-reporter mice with far-red fluorescent label

Authors

  • Y.V. Shebzukhov
  • A.A. Kuchmiy
  • A.A. Kruglov
  • F. Zipp
  • V. Siffrin
  • S.A. Nedospasov

Journal

  • Methods in Molecular Biology

Citation

  • Methods Mol Biol 1155: 151-162

Abstract

  • This chapter provides protocols for in vitro and in vivo analysis of TNF-producing cells from a novel TNF reporter mouse. In these transgenic mice, genetic sequence encoding far-red reporter protein Katyushka (FRFPK) was placed under control of the same regulatory elements as TNF, thus providing the basis for detection, isolation, and visualization of TNF-producing cells.


DOI

doi:10.1007/978-1-4939-0669-7_13