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Expression of MUC2-mucin in colorectal adenomas and carcinomas of different histological types

Authors

  • M. Blanke
  • E. Klussmann
  • S. Krueger-Krasagakes
  • A. Schmitt-Graeff
  • M. Stolte
  • G. Bornhoeft
  • H. Stein
  • P.X. Xing
  • I.F. McKenzie
  • C.P. Verstijnen
  • E.O. Riecken
  • C. Hanski

Journal

  • International Journal of Cancer

Citation

  • Int J Cancer 59 (3): 301-306

Abstract

  • The expression of mucin MUC2 was investigated in normal colonic tissue, in colonic adenomas and in carcinomas of the mucinous and non-mucinous type. The latter were subdivided into carcinomas originating from the adenoma-carcinoma sequence (ACS) and de novo (DN) carcinomas. The expression was assayed by immunohistochemistry with the monoclonal anti-MUC2 antibody CCP58 and by mRNA semiquantitation. MUC2 protein epitope CCP58 was strongly expressed in 21% of normal colonic tissues, in 40% of villous and in 48% of tubular adenomas. Mucinous carcinomas exhibited strong expression in 72%, ACS carcinomas in 21% and DN adenocarcinomas in none of the tumors investigated. Compared with the adjacent non-malignant tissue (transitional mucosa), CCP58 epitope expression in the tumor was higher in 74% of mucinous carcinomas, but equal or lower in 69% of ACS carcinomas and in 100% of de novo carcinomas. The alterations of MUC2 expression detected by immunohistochemistry in adenocarcinomas were confirmed on mRNA level. These data indicate that the MUC2 expression pattern is different in the 3 carcinoma types investigated. MUC2 over-expression occurs in the adenomatous tissue. It is always maintained in mucinous carcinomas, but frequently decreased in non-mucinous ACS carcinomas. DN carcinomas are most frequently associated with decreased expression of MUC2.


DOI

doi:10.1002/ijc.2910590302