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Expression, purification and preliminary X-ray crystallographic analysis of the chicken MHC class I molecule YF1*7.1

Authors

  • C.S. Hee
  • S. Gao
  • M.M. Miller
  • R.M. Goto
  • A. Ziegler
  • O. Daumke
  • B. Uchanska-Ziegler

Journal

  • Acta Crystallographica Section F

Citation

  • Acta Crystallogr Sect F Struct Biol Cryst Commun 65 (Pt 4): 422-425

Abstract

  • YF1*7.1 is an allele of a polymorphic major histocompatibility complex (MHC) class I-like locus within the chicken Y gene complex. With the aim of understanding the possible role of the YF1*7.1 molecule in antigen presentation, the complex of YF1*7.1 heavy chain and beta(2)-microglobulin was reconstituted and purified without a peptide. Crystals diffracted synchrotron radiation to 1.32 A resolution and belonged to the monoclinic space group P2(1). The phase problem was solved by molecular replacement. A detailed examination of the structure may provide insight into the type of ligand that could be bound by the YF1*7.1 molecule.


DOI

doi:10.1107/S1744309109009026