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Generation of Cre recombinase-specific monoclonal antibodies, able to characterize the pattern of Cre expression in cre-transgenic mouse strains

Authors

  • F. Schwenk
  • B. Sauer
  • N. Kukoč
  • R. Hoess
  • W. Müller
  • C. Kocks
  • R. Kühn
  • K. Rajewsky

Journal

  • Journal of Immunological Methods

Citation

  • J Immunol Methods 207 (2): 203-212

Abstract

  • Transgene-encoded Cre recombinase can target alteration of loxP-tagged genes to specific cell types and developmental stages in mice, depending on the pattern of transgene expression. To facilitate determination of the latter, we have generated monoclonal anti-Cre antibodies which are specific for distinct epitopes on the recombinase and detect Cre both on immunoblots and intracellularly by immunofluorescence. We demonstrate the usefulness of these antibodies by an analysis of Cre expression in mice carrying a cre-transgene under B cell-specific control.


DOI

doi:10.1016/S0022-1759(97)00116-6