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IL-10 supplementation increases Tregs and decreases hypertension in the RUPP rat model of preeclampsia

Authors

  • A. Harmon
  • D. Cornelius
  • L. Amaral
  • A. Paige
  • F. Herse
  • T. Ibrahim
  • G. Wallukat
  • J. Faulkner
  • J. Moseley
  • R. Dechend
  • B. LaMarca

Journal

  • Hypertension in Pregnancy

Citation

  • Hypertens Pregnancy 34 (3): 291-306

Abstract

  • OBJECTIVE: The reduced uterine perfusion pressure (RUPP) rat model of preeclampsia was used to determine the effects of added interleukin-10 (IL-10) on Tregs and hypertension in response to placental ischemia and how the decrease in these anti-inflammatory factors mediates the pathophysiology of preeclampsia. METHODS: IL-10 (2.5 ng/kg/d) was infused via osmotic mini-pump implanted intraperitoneally on day 14 of gestation and, at the same time, the RUPP procedure was performed. RESULTS: IL-10 reduced mean arterial pressure (p < 0.001), decreased CD4(+) T cells (p = 0.044), while increasing Tregs (p = 0.043) which led to lower IL-6 and TNF-alpha (p = 0.008 and p = 0.003), reduced AT1-AA production (p < 0.001), and decreased oxidative stress (p = 0.029) in RUPP rats. CONCLUSION: These data indicate that IL-10 supplementation increases Tregs and helps to balance the altered immune system seen during preeclampsia.


DOI

doi:10.3109/10641955.2015.1032054