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Inducible gene targeting in mice

Authors

  • R. Kühn
  • F. Schwenk
  • M. Aguet
  • K. Rajewsky

Journal

  • Science

Citation

  • Science 269 (5229): 1427-1429

Abstract

  • A method of gene targeting that allows the inducible inactivation of a target gene in mice is presented. The method uses an interferon-responsive promoter to control the expression of Cre recombinase. Here, Cre was used to delete a segment of the DNA polymerase beta gene flanked by IoxP recombinase recognition sites. Deletion was complete in liver and nearly complete in lymphocytes within a few days, whereas partial deletion was obtained in other tissues. This method can be used for the inducible inactivation of any other gene in vivo.


DOI

doi:10.1126/science.7660125