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Interpreting type 1 diabetes risk with genetics and single-cell epigenomics

Authors

  • J. Chiou
  • R.J. Geusz
  • M.L. Okino
  • J.Y. Han
  • M. Miller
  • R. Melton
  • E. Beebe
  • P. Benaglio
  • S. Huang
  • K. Korgaonkar
  • S. Heller
  • A. Kleger
  • S. Preissl
  • D.U. Gorkin
  • M. Sander
  • K.J. Gaulton

Journal

  • Nature

Citation

  • Nature 594 (7863): 398-402

Abstract

  • Genetic risk variants that have been identified in genome-wide association studies of complex diseases are primarily non-coding. Translating these risk variants into mechanistic insights requires detailed maps of gene regulation in disease-relevant cell types. Here we combined two approaches: a genome-wide association study of type 1 diabetes (T1D) using 520,580 samples, and the identification of candidate cis-regulatory elements (cCREs) in pancreas and peripheral blood mononuclear cells using single-nucleus assay for transposase-accessible chromatin with sequencing (snATAC-seq) of 131,554 nuclei. Risk variants for T1D were enriched in cCREs that were active in T cells and other cell types, including acinar and ductal cells of the exocrine pancreas. Risk variants at multiple T1D signals overlapped with exocrine-specific cCREs that were linked to genes with exocrine-specific expression. At the CFTR locus, the T1D risk variant rs7795896 mapped to a ductal-specific cCRE that regulated CFTR; the risk allele reduced transcription factor binding, enhancer activity and CFTR expression in ductal cells. These findings support a role for the exocrine pancreas in the pathogenesis of T1D and highlight the power of large-scale genome-wide association studies and single-cell epigenomics for understanding the cellular origins of complex disease.


DOI

doi:10.1038/s41586-021-03552-w