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Label-free detection of global morphology changes in confluent cell layers utilizing quantitative phase imaging with digital holographic microscopy

Authors

  • B. Kemper
  • L. Pohl
  • M. Kaiser
  • E. Döpker
  • J. Schnekenburger
  • S. Ketelhut

Journal

  • Proceedings of SPIE

Citation

  • Proc SPIE 11076: 30

Abstract

  • For the example of digital holographic microscopy (DHM), a variant of quantitative phase imaging (QPI), we explored strategies for the analysis of confluent cell layers utilizing histogram based-evaluation of quantitative phase images. The applicability of the proposed numerical evaluation procedures is illustrated by the DHM-based quantification of drug induced cell morphology changes. Our results show that histogram-based evaluation of quantitative phase images enables a highly reliable quantitative observation of global cellular morphology changes in confluent cell layers.


DOI

doi:10.1117/12.2527189