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Linescan microscopy data to extract diffusion coefficient of a fluorescent species using a commercial confocal microscope

Authors

  • M. Bathe-Peters
  • P. Gmach
  • P. Annibale
  • M.J. Lohse

Journal

  • Data in Brief

Citation

  • Data Brief 29: 105063

Abstract

  • We report here on the measurement of the diffusion coefficient of fluorescent species using a commercial microscope possessing a resonant scanner. Sequential linescans with a rate of up to 12 kHz yield a temporal resolution of 83 μs, making the setup amenable to measure diffusion rates over a range covering at least three orders of magnitude, from 100 μm(2)/s down to 0.1 μm(2)/s. We share representative data sets covering (i) the diffusion of a dye molecule, observed in media of different viscosities and (ii) the diffusion of a prototypical membrane receptor. The data can be valuable for researchers interested in the rapid diffusion properties of nuclear, cytosolic or membrane bound proteins fused to fluorescent tags.


DOI

doi:10.1016/j.dib.2019.105063