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Mapping the MHC class I spliced immunopeptidome of cancer cells

Authors

  • J. Liepe
  • J. Sidney
  • F.K.M. Lorenz
  • A. Sette
  • M. Mishto

Journal

  • Cancer Immunology Research

Citation

  • Cancer Immunol Res 7 (1): 62-76

Abstract

  • Anti-cancer immunotherapies demand optimal epitope targets, which could include proteasome-generated spliced peptides if tumor cells were to present them. Here, we show that spliced peptides are widely presented by MHC class I molecules of colon and breast carcinoma cell lines. The peptides derive from hot spots within antigens and enlarge the antigen coverage. Spliced peptides also represent a large number of antigens that would otherwise be neglected by patrolling T cells. These antigens tend to be long, hydrophobic, and basic. Thus, spliced peptides can be a key to identifying targets in an enlarged pool of antigens associated with cancer.


DOI

doi:10.1158/2326-6066.CIR-18-0424