- E. Kieback
- E. Hilgenberg
- S. Fillatreau
- Methods in Molecular Biology
- Methods Mol Biol 1193: 117-126
Retrogenic mice provide a unique system for rapidly analyzing the function of genes in the hematopoietic system. Here, we provide a detailed protocol for the production of retrogenic mice expressing genes coding for T cell receptor (TCR) for antigen. This technology should be easy to establish in any laboratory and should allow for a rapid progress in our understanding of the functional roles of TCR repertoires in immunity.