folder

A method for the generation of TCR retrogenic mice

Authors

  • E. Kieback
  • E. Hilgenberg
  • S. Fillatreau

Journal

  • Methods in Molecular Biology

Citation

  • Methods Mol Biol 1193: 117-126

Abstract

  • Retrogenic mice provide a unique system for rapidly analyzing the function of genes in the hematopoietic system. Here, we provide a detailed protocol for the production of retrogenic mice expressing genes coding for T cell receptor (TCR) for antigen. This technology should be easy to establish in any laboratory and should allow for a rapid progress in our understanding of the functional roles of TCR repertoires in immunity.


DOI

doi:10.1007/978-1-4939-1212-4_12