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MTCH2/MIMP is a major facilitator of tBID recruitment to mitochondria

Authors

  • Y. Zaltsman
  • L. Shachnai
  • N. Yivgi-Ohana
  • M. Schwarz
  • M. Maryanovich
  • R.H. Houtkooper
  • F.M. Vaz
  • F. De Leonardis
  • G. Fiermonte
  • F. Palmieri
  • B. Gillissen
  • P.T. Daniel
  • E. Jimenez
  • S. Walsh
  • C.M. Koehler
  • S.S. Roy
  • L. Walter
  • G. Hajnoczky
  • A. Gross

Journal

  • Nature Cell Biology

Citation

  • Nat Cell Biol 12 (6): 553-562

Abstract

  • The BH3-only BID protein (BH3-interacting domain death agonist) has a critical function in the death-receptor pathway in the liver by triggering mitochondrial outer membrane permeabilization (MOMP). Here we show that MTCH2/MIMP (mitochondrial carrier homologue 2/Met-induced mitochondrial protein), a novel truncated BID (tBID)-interacting protein, is a surface-exposed outer mitochondrial membrane protein that facilitates the recruitment of tBID to mitochondria. Knockout of MTCH2/MIMP in embryonic stem cells and in mouse embryonic fibroblasts hinders the recruitment of tBID to mitochondria, the activation of Bax/Bak, MOMP, and apoptosis. Moreover, conditional knockout of MTCH2/MIMP in the liver decreases the sensitivity of mice to Fas-induced hepatocellular apoptosis and prevents the recruitment of tBID to liver mitochondria both in vivo and in vitro. In contrast, MTCH2/MIMP deletion had no effect on apoptosis induced by other pro-apoptotic Bcl-2 family members and no detectable effect on the outer membrane lipid composition. These loss-of-function models indicate that MTCH2/MIMP has a critical function in liver apoptosis by regulating the recruitment of tBID to mitochondria.


DOI

doi:10.1038/ncb2057