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Polycomb repressive complex 2 is a barrier to KRAS-driven inflammation and epithelial-mesenchymal transition in non-small-cell lung cancer

Authors

  • M. Serresi
  • G. Gargiulo
  • N. Proost
  • B. Siteur
  • M. Cesaroni
  • M. Koppens
  • H. Xie
  • K.D. Sutherland
  • D. Hulsman
  • E. Citterio
  • S. Orkin
  • A. Berns
  • M. van Lohuizen

Journal

  • Cancer Cell

Citation

  • Canc Cell 29 (1): 17-31

Abstract

  • Polycomb repressive complexes (PRC) are frequently implicated in human cancer, acting either as oncogenes or tumor suppressors. Here, we show that PRC2 is a critical regulator of KRAS-driven non-small cell lung cancer progression. Modulation of PRC2 by either Ezh2 overexpression or Eed deletion enhances KRAS-driven adenomagenesis and inflammation, respectively. Eed-loss-driven inflammation leads to massive macrophage recruitment and marked decline in tissue function. Additional Trp53 inactivation activates a cell-autonomous epithelial-to-mesenchymal transition program leading to an invasive mucinous adenocarcinoma. A switch between methylated/acetylated chromatin underlies the tumor phenotypic evolution, prominently involving genes controlled by Hippo/Wnt signaling. Our observations in the mouse models were conserved in human cells. Importantly, PRC2 inactivation results in context-dependent phenotypic alterations, with implications for its therapeutic application.


DOI

doi:10.1016/j.ccell.2015.12.006