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Pop in, pop out: a novel gene-targeting strategy for use with CRISPR-Cas9

Authors

  • R. Kühn
  • V.T. Chu

Journal

  • Genome Biology

Citation

  • Genome Biol 16 (1): 244

Abstract

  • The CRISPR-Cas9 system is frequently used to create small deletions in the genomes of mammalian cells, but the isolation of precisely targeted mutants is still challenging. A new, two-step 'pop in & out' targeting approach facilitates this task.


DOI

doi:10.1186/s13059-015-0810-2