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The prevalence of TNFα-induced necrosis over apoptosis is determined by TAK1-RIP1 interplay

Authors

  • S. Coel Arslan
  • C. Scheidereit

Journal

  • PLoS ONE

Citation

  • PLoS ONE 6 (10): e26069

Abstract

  • Death receptor-induced programmed necrosis is regarded as a secondary death mechanism dominating only in cells that cannot properly induce caspase-dependent apoptosis. Here, we show that in cells lacking TGF{beta}-activated Kinase-1 (TAK1) expression, catalytically active Receptor Interacting Protein 1 (RIP1)-dependent programmed necrosis overrides apoptotic processes following Tumor Necrosis Factor-{alpha} (TNF{alpha}) stimulation and results in rapid cell death. Importantly, the activation of the caspase cascade and caspase-8-mediated RIP1 cleavage in TNF{alpha}-stimulated TAK1 deficient cells is not sufficient to prevent RIP1-dependent necrosome formation and subsequent programmed necrosis. Our results demonstrate that TAK1 acts independently of its kinase activity to prevent the premature dissociation of ubiquitinated-RIP1 from TNF{alpha}-stimulated TNF-receptor I and also to inhibit the formation of TNF{alpha}-induced necrosome complex consisting of RIP1, RIP3, FADD, caspase-8 and cFLIP(L). The surprising prevalence of catalytically active RIP1-dependent programmed necrosis over apoptosis despite ongoing caspase activity implicates a complex regulatory mechanism governing the decision between both cell death pathways following death receptor stimulation.


DOI

doi:10.1371/journal.pone.0026069