Quantitative phase imaging-based concepts for the analysis of global morphology changes in confluent cell layers
Authors
- B. Kemper
- L. Pohl
- M. Kaiser
- E. Doepker
- J. Schnekenburger
- S. Ketelhut
Journal
- Proceedings of SPIE
Citation
- Proc SPIE 10887: 108871M-1
Abstract
We have explored strategies for the analysis of confluent cell layers utilizing histogram based-evaluation of quantitative phase images for example of digital holographic microscopy (DHM), a variant of quantitative phase microscopy (QPM). The applicability of the proposed numerical procedures is illustrated by the DHM-based quantification of drug induced cell morphology changes. The achieved results show that histogram-based evaluation of quantitative phase images allows a highly reliable detection and continuous observation of global cellular morphology changes in confluent cell layers.