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Quantitative phase imaging-based concepts for the analysis of global morphology changes in confluent cell layers

Authors

  • B. Kemper
  • L. Pohl
  • M. Kaiser
  • E. Doepker
  • J. Schnekenburger
  • S. Ketelhut

Journal

  • Proceedings of SPIE

Citation

  • Proc SPIE 10887: 108871M-1

Abstract

  • We have explored strategies for the analysis of confluent cell layers utilizing histogram based-evaluation of quantitative phase images for example of digital holographic microscopy (DHM), a variant of quantitative phase microscopy (QPM). The applicability of the proposed numerical procedures is illustrated by the DHM-based quantification of drug induced cell morphology changes. The achieved results show that histogram-based evaluation of quantitative phase images allows a highly reliable detection and continuous observation of global cellular morphology changes in confluent cell layers.


DOI

doi:10.1117/12.2509242