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Restoration of uneven illumination in light sheet microscopy images

Authors

  • M.S. Uddin
  • H.K. Lee
  • S. Preibisch
  • P. Tomancak

Journal

  • Microscopy and Microanalysis

Citation

  • Microsc Microanal 17 (4): 607-613

Abstract

  • Light microscopy images suffer from poor contrast due to light absorption and scattering by the media. The resulting decay in contrast varies exponentially across the image along the incident light path. Classical space invariant deconvolution approaches, while very effective in deblurring, are not designed for the restoration of uneven illumination in microscopy images. In this article, we present a modified radiative transfer theory approach to solve the contrast degradation problem of light sheet microscopy (LSM) images. We confirmed the effectiveness of our approach through simulation as well as real LSM images.


DOI

doi:10.1017/S1431927611000262