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Structural and functional characterization of allatostatin receptor type-C of Thaumetopoea pityocampa, a potential target for next-generation pest control agents

Authors

  • A. Shahraki
  • A. Işbilir
  • B. Dogan
  • M.J. Lohse
  • S. Durdagi
  • N. Birgul-Iyison

Journal

  • Journal of Chemical Information and Modeling

Citation

  • J Chem Inf Model 61 (2): 715-728

Abstract

  • Insect neuropeptide receptors, including allatostatin receptor type C (AstR-C), a G protein-coupled receptor, are among the potential targets for designing next-generation pesticides that despite their importance in offering a new mode-of-action have been overlooked. Focusing on AstR-C of Thaumetopoea pityocampa, a common pest in Mediterranean countries, by employing resonance energy transfer-based methods, we showed Gαi/o coupling and β-arrestin recruitment of the receptor at sub-nanomolar and nanomolar ranges of the endogenous ligand, AST-C, respectively. Molecular docking and molecular dynamics simulation studies revealed the importance of extracellular loop 2 in AstRC/AST-C interaction, and a combination of in silico and in vitro approaches showed the substantial role of Q271(6.55) in G protein-dependent activation of AstR-C possibly via contributing to the flexibility of the receptor's structure. The functional and structural insights obtained on T. pit AstR-C positively assist future efforts in developing environmentally friendly pest control agents that are needed urgently.


DOI

doi:10.1021/acs.jcim.0c00985