Koronaler Schnitt

Rocks Lab

Spatio-temporal Control of Rho GTPase Signaling

Microscopes

Olympus Fluoview 1000 Confocal Microscope

Laser lines

405nm diode laser, 50mW

440nm diode laser, 25mW

Multi-Line Argon (458, 488, 515 nm)

515nm solid state laser, 25mW

559nm diode laser, 20mW

635nm diode laser, 20mW

Objectives

UPLSAPO 4X / 0.16 NA, WD 13 mm

UPLSAPO 10X / 0.4 NA, WD 3.1 mm

UPLSAPO 20X / 0.75 NA, WD 0.6 mm

UPLSAPO 40X / 0.95 NA, WD 0.18 mm, oil

UPLSAPO 60XS / 1.3 NA, WD 0.3 mm, silicone oil

LUCPLFLN 20X / 0.45 NA, long working distance (6.6 – 7.8 mm)

LUCPLFLN 40X / 0,6 NA, long working distance (2.7 - 4 mm)

Features

  • SIM Scanner - second scanning unit for simultaneous laser manipulation and imaging (FRAP, FLIP and photoactivation)
  • motorized stage (multi-area time-lapse imaging)
  • Incubation box to control temperature, humidity and CO2 (life cell imaging)
  • Z-drift compensation (ZDC, minimizes focus drift during time-lapse recording)
  • Eppendorf InjectMan NI 2 + FemtoJet (microinjection of fluorescent probes)

Olympus cell^R Imaging Station with cell^TIRF illuminator

Laser lines

405nm diode laser, 50mW

455nm diode laser, 50mW

488nm solid state laser, 150mW

514nm diode laser, 50mW

561nm solid state laser, 150mW

640nm diode laser, 140mW

TIRF Objective: UAPON 150X / 1.45 NA, WD 0.07 mm

Features

  • motorized 4 line TIRF system for simultaneous acquisition
  • motorized stage (multi-area time-lapse imaging)
  • Incubation box to control temperature, humidity and CO2 (life cell imaging)
  • Z-drift compensation (ZDC, minimizes focus drift during time-lapse recording)
  • Hamamatsu ImagEM EMCCD camera
  • Eppendorf InjectMan NI 2 + FemtoJet (microinjection of fluorescent probes)
  • PALM/dSTORM compatible

Picoquant Fluorescence Lifetime Imaging (FLIM) LSM upgrade

  • 485 nm pulse laser LDH-D-C-485
  • PicoHarp 300 TCSPC Module
  • Single Photon Counting Module (SPAD)
Dr. Oliver Rocks
Dr. Oliver Rocks
Contact
Phone: 3610
Max-Delbrück-Centrum für Molekulare Medizin (MDC)
Robert-Rössle-Str. 10
13092 Berlin, Deutschland
Building 31.1, Room 2029