Further information
(internal access only)
Secretariat
Max-Delbrück-Center for Molecular Medicine
Preclinical Research Center (PRC)
Robert-Rössle-Str. 10
13125 Berlin
phone: +49 30 9406 3521
This allows multiplex measurements to be performed, enabling several analytes to be determined simultaneously in the same sample volume.
The analytes are quantitatively fluorescently labeled and the emitted fluorescence is measured.
The required sample volume can be very small, often less than 5 µl, maximum 50µl.
For example, a standard assay can be run on a 96-well plate and is designed to analyze 80 serum or plasma samples. The multiplex analyzer used at the APP, Luminex xMAP Intelliflex DR-SE, offers the ability to measure in 96-well plates as well as in the large 384-well approach.
Commercially available assays, specific for mouse and rat, among others, are available for a variety of analytes and address research fields e.g. in immunology, cancer, cardiovascular, metabolism, development and many more.
In the multiplex approach, the targeted proteins are immunologically coupled to color-labeled beads. The use of classification lasers (bead lasers) and reporter lasers enables the simultaneous identification of proteins and their respective quantification. The laser optics ensure highest precision and data density of the measurements.
Several established assays from different vendors are available for mouse and rat, e.g.:
Mouse Cytokine/Chemokine Panel (32-plex):
Eotaxin/CCL11 | IL-1β | G-CSF | GM-CSF | IFNγ | IL-1α | IL-1β | IL-2 | IL-3 | IL-4 | IL-5 | IL-6 | IL-7 | IL-9 | IL-10 | IL-12 (p40) | IL-12 (p70) | IL-13 | IL-15 | IL-17A/CTLA8 | IP-10/CXCL10 | KC/GRO/CINC-1/CXCL1 | LIF | LIX | MCP-1/CCL2 | M-CSF | MIG/CXCL9 | MIP-1α/CCL3 | MIP-1β/CCL4 | MIP-2/CXCL2 | RANTES/CCL5 | TNFα | VEGF-A
Mouse Cardiovascular Panel (9-plex):
sCD40L | CXCL16 | Endocan-1 | Follistatin | LIGHT | Oncostatin M | Placental Growth Factor 2 | TnI | TnT
Mouse Adipokine (7-plex)
IL-6 | Insulin | Leptin | MCP-1/CCL2 | PAI-1 | Resistin | TNFα
Mouse/Rat Pituitary Gland (7-plex):
ACTH | BDNF | FSH | GH | LH | Prolactin | TSH
Rat Stress Hormone (3-plex):
ACTH | Corticosterone | Melatonin
Rat Bone Panel (8-Plex):
ACTH | DKK1 | FGF-23 | Insulin | Leptin | Osteoprotegerin | PTH | Sclerostin