Rapid generation of inducible mouse mutants
Autor/innen
- J. Seibler
- B. Zevnik
- B. Küter-Luks
- S. Andreas
- H. Kern
- T. Hennek
- A. Rode
- C. Heimann
- N. Faust
- G. Kauselmann
- M. Schoor
- R. Jaenisch
- K. Rajewsky
- R. Kühn
- F. Schwenk
Journal
- Nucleic Acids Research
Quellenangabe
- Nucleic Acids Res 31 (4): e12
Zusammenfassung
We have generated an optimized inducible recombination system for conditional gene targeting based on a Cre recombinase-steroid receptor fusion. This configuration allows efficient Cre-mediated recombination in most organs of the mouse upon induction, without detectable background activity. An ES cell line, was established that carries the inducible recombinase and a loxP-flanked lacZ reporter gene. Out of this line, completely ES cell-derived mice were efficiently produced through tetraploid blastocyst complementation, without the requirement of mouse breeding. Our findings provide a new concept allowing the generation of inducible mouse mutants within 6 months, as compared to 14 months using the current protocol.