In vivo stable isotope labeling by amino acids in Drosophila melanogaster
Autor/innen
- M.D. Sury
- J.X. Chen
- M. Selbach
Journal
- Methods in Molecular Biology
Quellenangabe
- Methods Mol Biol 1188: 85-93
Zusammenfassung
The fruit fly Drosophila melanogaster is one of the most widely used and well-studied model organisms in biology and therefore a promising tool for quantitative proteomics. Here, we describe a method to label D. melanogaster with stable isotope labeled amino acids in vivo. Feeding flies with heavy lysine labeled yeast cells leads to virtually complete heavy labeling already in the first filial generation. The approach is simple, fast, and cost-effective, which makes SILAC flies an attractive model system for the emerging field of in vivo quantitative proteomics.