© Katharina Bohm / MDC
Confocal and Two-Photon Microscopy
Our confocal microscopes are equipped with multiple laser lines, various objectives and spectral detectors (PMTs or high sensitivity detectors). Two-photon excitation is possible for thick samples. Observation of subcellular structures can be done with the high-resolution Airy scan detector. A dedicated FLIM microscope can be used to study intracellular protein-protein interactions.
More information is available for internal users here.
- Instruments
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A list of our confocal microscopes is shown below (click here).
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Leica TCS SPE
Leica TCS SP5
Leica TCS SP8
Zeiss LSM 700
Zeiss LSM 710
Zeiss LSM 880 Airy scan
Nikon/Andor CSU-W1
Becker & Hickl FLIM
- Some applications
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A list of potential applications is shown below (click here).
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- Sequential multi-color fluorescence imaging (UV/green/red/far-red dyes) of adherent fixed cells and tissue sections up to 100 µm thickness
- z-stacks for 3D reconstructions
- Tile scans for large area samples
- Multiposition imaging
- Spectral unmixing of overlapping emission spectra
- Live cell imaging with very fast time series (e.g. vesicle movement, Ca2+responses)
- Long-term live imaging of adherent cells
- FRET (Förster resonant energy transfer) for detecting protein-protein interactions in situ measured via sensitized emission (fluorescence intensity ratio) or fluorescence lifetime imaging (FLIM)
- Laser photomanipulations such as FRAP (fluorescence recovery after photobleaching) or photoswitching/ photoactivation
- Confocal imaging with Airyscan technology (about two-fold improved resolution, speed and sensitivity)
- 2-Photon excitation for imaging in thick samples
- (Intravital) imaging of small organs/organisms with long working distance objective and/or 2-Photon excitation and non-descanned detection
- DIC microscopy