Superresolution & TIRF microscopy

Breaking the resolution limit is possible using several microscopy techniques. We offer wide field TIRF microscopy for studying dynamic membrane processes in live cells and possible applications to localisation microscopy (e.g. PALM). Our STED microscope can be used for both fixed and live samples as well as for the RESOLFT technique.

More information is available for internal users here.

Instruments

A list of our superresolution and TIRF microscopes is shown below (click here).

• 3i Vector TIRF

• Olympus TIRF

• Abberior STED

Some TIRF applications

A list of potential applications is shown below (click here).

• observation of membrane-associated cellular processes

• FRET microscopy by sensitized emission

• fast, sensitive single molecule imaging and tracking

Some STED applications

A list of potential applications is shown below (click here).

• subdiffraction-resolution imaging of subcellular structures and organelles in membranes, cytoplasm and nucleus of flat adherent cells and thin tissue sections.

• multicolor superresolution imaging