The IκB kinase complex and NF-κB act as master regulators of lipopolysaccharide-induced gene expression and control subordinate activation of AP-1


  • D. Krappmann
  • E. Wegener
  • Y. Sunami
  • M. Esen
  • A. Thiel
  • B. Mordmueller
  • C. Scheidereit


  • Molecular and Cellular Biology


  • Mol Cell Biol 24 (14): 6488-6500


  • Toll-like receptors (TLRs) recognize conserved products of microbial pathogens to initiate the innate immune response. TLR4 signaling is triggered upon binding of lipopolysaccharides (LPS) from gram-negative bacteria. Using comparative gene expression profiling, we demonstrate a master regulatory role of I{kappa}B kinase (IKK)/NF-{kappa}B signaling for immediate-early gene induction after LPS engagement in precursor B cells. IKK/NF-{kappa}B signaling controls a large panel of gene products associated with signaling and transcriptional activation and repression. Intriguingly, the induction of AP-1 activity by LPS in precursor B cells and primary dendritic cells fully depends on the IKK/NF-{kappa}B pathway, which promotes expression of several AP-1 family members, including JunB, JunD, and B-ATF. In pre-B cells, AP-1 augments induction of a subset of primary NF-{kappa}B targets, as shown for chemokine receptor 7 (CCR7) and immunoglobulin K light chain. Thus, our data illustrate that NF-{kappa}B orchestrates immediate-early effects of LPS signaling and controls secondary AP-1 activation to mount an appropriate biological response.