The IκB kinase complex and NF-κB act as master regulators of lipopolysaccharide-induced gene expression and control subordinate activation of AP-1
Authors
- D. Krappmann
- E. Wegener
- Y. Sunami
- M. Esen
- A. Thiel
- B. Mordmueller
- C. Scheidereit
Journal
- Molecular and Cellular Biology
Citation
- Mol Cell Biol 24 (14): 6488-6500
Abstract
Toll-like receptors (TLRs) recognize conserved products of microbial pathogens to initiate the innate immune response. TLR4 signaling is triggered upon binding of lipopolysaccharides (LPS) from gram-negative bacteria. Using comparative gene expression profiling, we demonstrate a master regulatory role of I{kappa}B kinase (IKK)/NF-{kappa}B signaling for immediate-early gene induction after LPS engagement in precursor B cells. IKK/NF-{kappa}B signaling controls a large panel of gene products associated with signaling and transcriptional activation and repression. Intriguingly, the induction of AP-1 activity by LPS in precursor B cells and primary dendritic cells fully depends on the IKK/NF-{kappa}B pathway, which promotes expression of several AP-1 family members, including JunB, JunD, and B-ATF. In pre-B cells, AP-1 augments induction of a subset of primary NF-{kappa}B targets, as shown for chemokine receptor 7 (CCR7) and immunoglobulin K light chain. Thus, our data illustrate that NF-{kappa}B orchestrates immediate-early effects of LPS signaling and controls secondary AP-1 activation to mount an appropriate biological response.