Best practice standards for circular RNA research


  • A.F. Nielsen
  • A. Bindereif
  • I. Bozzoni
  • M. Hanan
  • T.B. Hansen
  • M. Irimia
  • S. Kadener
  • L.S. Kristensen
  • I. Legnini
  • M. Morlando
  • M.T. Jarlstad Olesen
  • R.J. Pasterkamp
  • S. Preibisch
  • N. Rajewsky
  • C. Suenkel
  • J. Kjems


  • Nature Methods


  • Nat Methods 19 (10): 1208-1220


  • Circular RNAs (circRNAs) are formed in all domains of life and via different mechanisms. There has been an explosion in the number of circRNA papers in recent years; however, as a relatively young field, circRNA biology has an urgent need for common experimental standards for isolating, analyzing, expressing and depleting circRNAs. Here we propose a set of guidelines for circRNA studies based on the authors' experience. This Perspective will specifically address the major class of circRNAs in Eukarya that are generated by a spliceosome-catalyzed back-splicing event. We hope that the implementation of best practice principles for circRNA research will help move the field forward and allow a better functional understanding of this fascinating group of RNAs.