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CCR7 on CD4(+) T cells plays a crucial role in the induction of experimental autoimmune encephalomyelitis

Authors

  • P. Belikan
  • U. Bühler
  • C. Wolf
  • G.K. Pramanik
  • R. Gollan
  • F. Zipp
  • V. Siffrin

Journal

  • Journal of Immunology

Citation

  • J Immunol 200 (8): 2554-2562

Abstract

  • Multiple sclerosis (MS) is the most common chronic inflammatory demyelinating disease of the CNS. Myelin-specific CD4(+) Th lymphocytes are known to play a major role in both MS and its animal model experimental autoimmune encephalomyelitis (EAE). CCR7 is a critical element for immune cell trafficking and recirculation, that is, lymph node homing, under homeostatic conditions; blocking CCR7(+) central memory cells from egress of lymph nodes is a therapeutic approach in MS. To define the effect of CD4(+) T cell-specific constitutive deletion of CCR7 in the priming and effector phase in EAE, we used an active EAE approach in T cell reconstituted Rag1(-/-) mice, as well as adoptive transfer EAE, in which mice received in vitro-primed CCR7(-/-) or CCR7(+/+) myelin Ag TCR-transgenic 2d2 Th17 cells. Two-photon laser scanning microscopy was applied in living anesthetized mice to monitor the trafficking of CCR7-deficient and wild-type CD4(+) T cells in inflammatory lesions within the CNS. We demonstrate that CD4(+) T cell-specific constitutive deletion of CCR7 led to impaired induction of active EAE. In adoptive transfer EAE, mice receiving in vitro-primed CCR7(-/-) 2d2 Th17 cells showed similar disease onset as mice adoptively transferred with CCR7(+/+) 2d2 Th17 cells. Using two-photon laser scanning microscopy CCR7(-/-) and CCR7(+/+) CD4(+) T cells did not reveal differences in motility in either animal model of MS. These findings indicate a crucial role of CCR7 in neuroinflammation during the priming of autoimmune CD4(+) T cells but not in the CNS.


DOI

doi:10.4049/jimmunol.1701419