Excess NF-κB induces ectopic odontogenesis in embryonic incisor epithelium


  • J. Blackburn
  • K. Kawasaki
  • T. Porntaveetus
  • M. Kawasaki
  • Y. Otsuka-Tanaka
  • Y. Miake
  • M.S. Ota
  • M. Watanabe
  • M. Hishinuma
  • T. Nomoto
  • S. Oommen
  • S. Ghafoor
  • F. Harada
  • K. Nozawa-Inoue
  • T. Maeda
  • R. Peterkova
  • H. Lesot
  • J. Inoue
  • T. Akiyama
  • R. Schmidt-Ullrich
  • B. Liu
  • Y. Hu
  • A. Page
  • A. Ramirez
  • P.T. Sharpe
  • A. Ohazama


  • Journal of Dental Research


  • J Dent Res 94 (1): 121-128


  • Nuclear factor κB (NF-κB) signaling plays critical roles in many physiological and pathological processes, including regulating organogenesis. Down-regulation of NF-κB signaling during development results in hypohidrotic ectodermal dysplasia. The roles of NF-κB signaling in tooth development, however, are not fully understood. We examined mice overexpressing IKK{beta}, an essential component of the NF-κB pathway, under keratin 5 promoter (K5-Ikkβ). K5-Ikkβ mice showed supernumerary incisors whose formation was accompanied by up-regulation of canonical Wnt signaling. Apoptosis that is normally observed in wild-type incisor epithelium was reduced in K5-Ikkβ mice. The supernumerary incisors in K5-Ikkβ mice were found to phenocopy extra incisors in mice with mutations of Wnt inhibitor, Wise. Excess NF-κB activity thus induces an ectopic odontogenesis program that is usually suppressed under physiological conditions.