Genome editing in mice using TALE nucleases
Authors
- B. Wefers
- C. Brandl
- O. Ortiz
- W. Wurst
- R. Kühn
Journal
- Methods in Molecular Biology
Citation
- Methods Mol Biol 1338: 229-243
Abstract
Gene engineering for generating targeted mouse mutants is a key technology for biomedical research. Using TALENs as sequence-specific nucleases to induce targeted double-strand breaks, the mouse genome can be directly modified in zygotes in a single step without the need for embryonic stem cells. By embryo microinjection of TALEN mRNAs and targeting vectors, knockout and knock-in alleles can be generated fast and efficiently. In this chapter we provide protocols for the application of TALENs in mouse zygotes.