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The hyperactive Sleeping Beauty transposase SB100X improves the genetic modification of T cells to express a chimeric antigen receptor

Authors

  • Z. Jin
  • S. Maiti
  • H. Huls
  • H. Singh
  • S. Olivares
  • L. Mates
  • Z. Izsvak
  • Z. Ivics
  • D.A. Lee
  • R.E. Champlin
  • L.J. Cooper

Journal

  • Gene Therapy

Citation

  • Gene Ther 18 (9): 849-856

Abstract

  • Sleeping Beauty (SB(3)) transposon and transposase constitute a DNA plasmid system used for therapeutic human cell genetic engineering. Here we report a comparison of SB100X, a newly developed hyperactive SB transposase, to a previous generation SB11 transposase to achieve stable expression of a CD19-specific chimeric antigen receptor (CAR(3)) in primary human T cells. The electro-transfer of SB100X expressed from a DNA plasmid or as an introduced mRNA species had superior transposase activity in T cells based on the measurement of excision circles released after transposition and emergence of CAR expression on T cells selectively propagated upon CD19(+) artificial antigen-presenting cells. Given that T cells modified with SB100X and SB11 integrate on average one copy of the CAR transposon in each T-cell genome, the improved transposition mediated by SB100X apparently leads to an augmented founder effect of electroporated T cells with durable integration of CAR. In aggregate, SB100X improves SB transposition in primary human T cells and can be titrated with an SB transposon plasmid to improve the generation of CD19-specific CAR(+) T cells.


DOI

doi:10.1038/gt.2011.40