Two-Photon microscopy for in vivo deep tissue imaging

Deep tissue and organ imaging is possible due to the high penetration depth of the infrared radiation in Two-Photon microscopy. Our dedicated setup is completed with equipment for maintaining conditions for long-term live imaging. Large area imaging is possible for cleared or un-cleared tissue samples.

More information is available for internal users here.

Instrument

(click here)

LaVision Biotec Trimscope II

Some applications

A list of potential applications is shown below (click here).

• Two-photon fluorescence imaging for non-transparent tissues labelled with fluorescent proteins, selected fluorophores or quantum dots using up to three different excitation wavelengths and six detection channels simultaneously and possibilities for spectral unmixing

• in-vivo fluorescence imaging of cells expressing fluorescent proteins and/​or of blood vessels labelled with fluorescent dextranes or quantum dots 

• SHG (second harmonic generation) of collagen fibres

• Functional imaging of intracellular signals such as Ca²⁺ activation

• FRET imaging of endogenously expressed biosensors via sensitized emission (fluorescence intensity ratio) or FLIM (fluorescence lifetime imaging)

• Label free imaging of cellular autofluorescence (NADH, FAD)

• Photoactivation of bacteriorhodpsins for optogenetics studies

• Tile scans and multiposition imaging