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Leutz Lab

Cell Differentiation and Tumorigenesis

Profile

We study the development of blood cells and the emergence of leukemia by focusing on the molecular genetics of self-renewal and its connection to differentiation into functional cells of the innate immune system, like macrophages, granulocytes, and dendritic cells.

The inherent self-renewal property of hematopoietic stem cells vanishes during differentiation but may reoccur in tumor cells (see “Research” for details;). Key gene regulatory proteins control cell multiplication and instruct cells to adopt distinct cell fates. Dysregulation of these master switches may affect differentiation, which can lead to “confused” cells as the potential progenitors of leukemia. We explore molecular mechanism that govern these processes in homeostasis and during disease.

Currently, we concentrate on the functions of “pioneering transcription factors” CCAAT Enhancer Binding Proteins alpha and beta (C/EBPα,β) in development and disease. Both C/EBPs command and control molecular programs of proliferation and differentiation into innate immune cells, including various types of myeloid cells. Previously, we have shown that dysregulation of the WNT-signaling pathway and interference with the transcription factor IRF8 are major deregulated pathways in chronic myelogenous leukemia (CML).

We apply targeted mouse genetics in combination with experimental hematopoiesis, genetic engineering of hematopoietic cells, human iPSC hematopoietic differentiation, proteomics and biochemistry. Together with the associated research group of Dr. Alexander Mildner we perform ATACseq, ChIPseq and single cell analysis to disclose fundamental epigenetic mechanisms in hematopoiesis, homeostasis, autoimmune diseases, and in leukemogenesis.

 

 

Team

Research

Hematopoietic stem cells in the bone marrow sustain their own maintenance and give rise to “transit amplifying”, lineage committed cells that terminally differentiate into distinct, mostly short-lived cell types. Hematopoietic transcription factors (= gene regulatory proteins) concertedly control cell proliferation, lineage commitment, and cell differentiation. Mutations in these key transcription factors dysregulate hematopoiesis and cause diseases, such as leukemia and immune defects. Experimental hematology, leukemia research, in combination with stem cell research strives to untangle the underlying “grammar” of transcription factor networks.

We disclose molecular and functional interactions between regulatory proteins and determine how they orchestrate the complex process of proper gene expression and thereby reveal novel therapeutic targets. The lab currently focuses on functions of transcription factors of the CCAAT Enhancer Binding Protein family (C/EBPs). We use state-of-the-art molecular genetics, targeted mouse genetics, genomic approaches and proteomics to unravel how C/EBPs connect to innate immunity and onco-developmental transformation.

Publications

Prof. Dr. Achim Leutz
Prof. Dr. Achim Leutz
Group Leader
Contact
Max-Delbrück-Centrum für Molekulare Medizin (MDC)
Robert-Rössle-Str. 10
13092 Berlin, Germany
31.1: Max Delbrück House
Room 4028
(030) 9406 3735
 
Secretariat
 
Sylvia Sibilak
(030) 9406 3349
31.1: Max Delbrück House
Room 4029